BACKGROUND AND OBJECTIVES

Antibiotic resistance in microorganisms is a significant global health concern. Cold atmospheric plasma is an innovative and promising method for inactivating bacteria. This study aimed to evaluate the effects of cold plasma on in a mouse wound infection model.

MATERIALS AND METHODS

The disk diffusion method was used to perform antibiograms after isolating the bacteria. A multidrug-resistant strain was then selected. The bactericidal activity of cold helium plasma was investigated in vitro. The optimal cold plasma conditions were determined in the laboratory, with a flow of 3 liters per minute and a power of 1.1 watts. These conditions were later used for in vivo evaluations.

RESULTS

In a laboratory study, helium gas plasma treatment for 8 minutes reduced by 2.5 logs. In the in vivo study, plasma reduced the wound's microbial load in mice by 1.9 log. The antibiotic treatment group had a 1.2 log reduction. Both plasma and antibiotic therapies had similar effects on microbial inactivation.

CONCLUSION

The overall evaluation of wound healing time and pathological features showed that plasma was generally better than antibiotic treatment. Plasma can inactivate in wounds and accelerate wound healing.