Department of Biotechnology, Engineering School of Lorena, University of São Paulo, Brazil.
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, Brazil.
Bioresour Technol. 2020 Oct;314:123716. doi: 10.1016/j.biortech.2020.123716. Epub 2020 Jun 27.
β-Glucans as emerging biopolymer are widely produced by microorganisms in fermentation processes using commercial sugars which make process non-economic. Lignocellulosic substances are inexpensive carbon sources, which could be exploited for sustainable production of β-glucans. In this study, a lignocellulosic material, namely sugarcane straw (SCS) was utilized for the production of extracellular β-glucan by Lasiodiplodia theobromae CCT3966. SCS was subjected to acid and subsequent alkaline pretreatment, followed by enzymatic saccharification using cellulase enzyme. Quantity of 48.65 g/L glucose was released after enzymatic hydrolysis. β-Glucan production was performed by cultivation of fungal strain in SCS hydrolysate at 28 °C and initial culture pH 7. Highest β-glucan yield and productivity of 0.047 gg and 0.014 gLh, respectively was obtained at 72 h fermentation time. Kinetic study of β-glucan production revealed experimental biosynthesis of β-glucan from SCS hydrolysate followed the trend generated by Logistic and Luedeking-Piret models. Chemical structure of biopolymer produced showed β-glucan constitution.
β-葡聚糖作为一种新兴的生物聚合物，广泛存在于微生物的发酵过程中，可以利用商业糖来生产，这使得生产过程不具有经济性。木质纤维素类物质是一种廉价的碳源，可以用来可持续生产β-葡聚糖。在本研究中，利用 Lasiodiplodia theobromae CCT3966 从一种木质纤维素材料，即甘蔗渣（SCS）中生产胞外β-葡聚糖。对 SCS 进行酸预处理和后续的碱预处理，然后用纤维素酶进行酶解糖化。酶解后释放出 48.65g/L 的葡萄糖。在 28°C 和初始培养 pH 值为 7 的条件下，将真菌菌株在 SCS 水解物中培养，进行β-葡聚糖生产。在 72 小时发酵时间下，获得了最高的β-葡聚糖产量和生产力，分别为 0.047 gg 和 0.014 gLh。β-葡聚糖生产的动力学研究表明，从 SCS 水解物中生物合成β-葡聚糖的实验符合 Logistic 和 Luedeking-Piret 模型的趋势。所生产的生物聚合物的化学结构表明其为β-葡聚糖结构。
