Guillén Alis, Granados Sergio, Rivas Kevin Eduardo, Estrada Omar, Echeverri Luis Fernando, Balcázar Norman
Molecular Genetics Group, University of Antioquia, Calle 70, No. 52-21, A.A. 1226, Medellín, Colombia.
Department of Physiology and Biochemistry, School of Medicine, University of Antioquia, Calle 70, No. 52-21, A.A. 1226, Medellín, Colombia.
Adv Pharmacol Sci. 2015;2015:418673. doi: 10.1155/2015/418673. Epub 2015 Aug 20.
Eucalyptus tereticornis is a plant used in traditional medicine to control diabetes, but this effect has not been proved scientifically. Here, we demonstrated through in vitro assays that E. tereticornis extracts increase glucose uptake and inhibit their production in insulin-resistant C2C12 and HepG2 cells, respectively. Furthermore, in a nutritional model using diabetic mice, the administration of ethyl acetate extract of E. tereticornis reduced fasting glycaemia, improved tolerance to glucose, and reduced resistance to insulin. Likewise, this extract had anti-inflammatory effects in adipose tissue when compared to control diabetic mice. Via bioguided assays and sequential purification of the crude extract, a triterpenoid-rich fraction from ethyl acetate extracts was shown to be responsible for the biological activity. Similarly, we identified the main compound responsible for the antihyperglycemic activity in this extract. This study shows that triterpenes found in E. tereticornis extracts act as hypoglycemic/antidiabetic compounds and contribute to the understanding of their use in traditional medicine.
棱果桉是一种在传统医学中用于控制糖尿病的植物，但这种效果尚未得到科学证明。在此，我们通过体外试验证明，棱果桉提取物分别增加胰岛素抵抗的C2C12和HepG2细胞中的葡萄糖摄取并抑制其生成。此外，在使用糖尿病小鼠的营养模型中，给予棱果桉乙酸乙酯提取物可降低空腹血糖、改善葡萄糖耐受性并降低胰岛素抵抗。同样，与对照糖尿病小鼠相比，该提取物在脂肪组织中具有抗炎作用。通过生物导向试验和粗提物的顺序纯化，乙酸乙酯提取物中富含三萜类化合物的部分被证明具有生物活性。同样，我们鉴定出了该提取物中负责降血糖活性的主要化合物。这项研究表明，棱果桉提取物中的三萜类化合物具有降血糖/抗糖尿病作用，并有助于理解其在传统医学中的应用。
