Kang Ran, Li Haisheng, Lysdahl Helle, Quang Svend Le Dang, Chen Menglin, Xie Lin, Bünger Cody
Orthopaedic Research Lab, Aarhus University Hospital, Aarhus, C 8000, Denmark.
Department of Orthopedic Surgery, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, 210028, China.
J Biomed Mater Res B Appl Biomater. 2017 Jan;105(1):14-20. doi: 10.1002/jbm.b.33524. Epub 2015 Sep 15.
In an attempt to find an ideal closure method during annulus defect repair, we evaluate the use of medical glue by mechanical and biocompatible test. Cyanoacrylate medical glue was applied together with a multilayer microfiber/nanofiber polycaprolactone scaffold and suture in annulus repair. Continuous axial loading and fatigue mechanical test was performed. Furthermore, the in vitro response of mesenchymal stem cell (MSC) to the glue was evaluated by cell viability assay. The in vivo response of annulus tissue to the glue and scaffold was also studied in porcine lumbar spine; histological sections were evaluated after 3 months. Cyanoacrylate glue significantly improved the closure effect in the experimental group with failure load 2825.7 ± 941.6 N, compared to 774.1 ± 281.3 N in the control group without glue application (p < 0.01). The experimental group also withstood the fatigue test. No toxic effect was observed by in vitro cell culture and in vivo implantation. On the basis of this initial evaluation, the use of cyanoacrylate medical glue improves closure effect with no toxicity in annulus defect repair. This method of annulus repair merits further effectiveness study in vivo. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 14-20, 2017.
为了在纤维环缺损修复过程中找到一种理想的闭合方法，我们通过力学和生物相容性测试评估医用胶水的使用情况。在纤维环修复中，将氰基丙烯酸酯医用胶水与多层微纤维/纳米纤维聚己内酯支架及缝线一起应用。进行了连续轴向加载和疲劳力学测试。此外，通过细胞活力测定评估间充质干细胞（MSC）对胶水的体外反应。还在猪腰椎中研究了纤维环组织对胶水和支架的体内反应；3个月后评估组织学切片。与未使用胶水的对照组（774.1±281.3 N）相比，氰基丙烯酸酯胶水显著提高了实验组的闭合效果，失败负荷为2825.7±941.6 N（p<0.01）。实验组也经受住了疲劳测试。体外细胞培养和体内植入均未观察到毒性作用。基于这一初步评估，氰基丙烯酸酯医用胶水在纤维环缺损修复中提高了闭合效果且无毒性。这种纤维环修复方法值得在体内进行进一步的有效性研究。©2015威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，105B：14 - 20，2017。
