Hansz J, Sawiński K, Woźny T
Acta Haematol Pol. 1989 Jul-Dec;20(2):133-9.
The humoral effect of resting and phorbol myristate acetate (PMA) preincubated normal monocytes on granulocytic progenitor growth in double diffusion chamber culture (CFU-dG) was assayed. The activation of monocytes by PMA was confirmed in NBT reduction test (increased formazan content) and by enhanced superoxide anions production compared with resting cells. The activatory and inhibitory effect of monocytes on granulocytic colony formation was cell-concentration dependent. The maximal CFU-dG growth promoting effects was seen at 5 X 10(4) of resting monocytes, whereas PMA-treated cells exerted their optimal stimulatory activity at lower cell number (2.5 X 10(4)). Further increase of monocyte concentration resulted in diminished CFU-dG proliferation. It is shown that the decline of colony count at high monocyte number was caused by indomethacin-dependent inhibitors.
检测了静息及经佛波酯（PMA）预孵育的正常单核细胞在双扩散小室培养（CFU-dG）中对粒细胞祖细胞生长的体液效应。通过NBT还原试验（甲臜含量增加）以及与静息细胞相比超氧阴离子产生增强，证实了PMA对单核细胞的激活作用。单核细胞对粒细胞集落形成的激活和抑制作用取决于细胞浓度。静息单核细胞数量为5×10⁴时，CFU-dG生长促进作用最大，而经PMA处理的细胞在较低细胞数量（2.5×10⁴）时发挥最佳刺激活性。单核细胞浓度进一步增加导致CFU-dG增殖减少。结果表明，单核细胞数量较高时集落数的下降是由吲哚美辛依赖性抑制剂引起的。
