Roxbury Daniel, Jena Prakrit V, Williams Ryan M, Enyedi Balázs, Niethammer Philipp, Marcet Stéphane, Verhaegen Marc, Blais-Ouellette Sébastien, Heller Daniel A
Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
Weill Cornell Medical College, New York, NY, USA.
Sci Rep. 2015 Sep 21;5:14167. doi: 10.1038/srep14167.
The intrinsic near-infrared photoluminescence (fluorescence) of single-walled carbon nanotubes exhibits unique photostability, narrow bandwidth, penetration through biological media, environmental sensitivity, and both chromatic variety and range. Biomedical applications exploiting this large family of fluorophores will require the spectral and spatial resolution of individual (n,m) nanotube species' fluorescence and its modulation within live cells and tissues, which is not possible with current microscopy methods. We present a wide-field hyperspectral approach to spatially delineate and spectroscopically measure single nanotube fluorescence in living systems. This approach resolved up to 17 distinct (n,m) species (chiralities) with single nanotube spatial resolution in live mammalian cells, murine tissues ex vivo, and zebrafish endothelium in vivo. We anticipate that this approach will facilitate multiplexed nanotube imaging in biomedical applications while enabling deep-tissue optical penetration, and single-molecule resolution in vivo.
单壁碳纳米管的本征近红外光致发光（荧光）具有独特的光稳定性、窄带宽、可穿透生物介质、对环境敏感，以及具有多种颜色和光谱范围。利用这一大类荧光团的生物医学应用将需要对单个（n,m）纳米管种类的荧光进行光谱和空间分辨率分析，并在活细胞和组织中对其进行调制，而目前的显微镜方法无法做到这一点。我们提出了一种宽场高光谱方法，用于在空间上描绘和光谱测量活体系统中的单个纳米管荧光。这种方法在活的哺乳动物细胞、离体小鼠组织和体内斑马鱼内皮中，以单纳米管空间分辨率分辨出多达17种不同的（n,m）种类（手性）。我们预计，这种方法将有助于生物医学应用中的多重纳米管成像，同时实现深层组织的光学穿透和体内单分子分辨率。
