Peristiowati Yuly, Indasah Indasah, Ratnawati Retty
Department of Nurse, Surya Mitra Husada Health School, Kediri, East Java, Indonesia.
Department of Public Health, Surya Mitra Husada Health School, Kediri, East Java, Indonesia.
J Intercult Ethnopharmacol. 2015 Apr-Jun;4(2):114-7. doi: 10.5455/jice.20141224104135. Epub 2015 Mar 3.
This study aimed to investigate whether a catechin isolated from GMB-4 green tea is able to increase the reducing equivalent system and nitric oxide (NO) level in endothelial cells exposed to high glucose (HG) level.
Endothelial cells were obtained from human umbilical vascular tissues. At confluent, human endothelial cells were divided into five groups, which included control (untreated), endothelial cells exposed to HG (30 mM), endothelial cells exposed to HG in the presence of green tea catechin (HG + C) at the following three doses: 0.03; 0.3; and 3 mg/ml. Analysis of NADP(+), NADPH, and NO levels were performed colorimetrically.
This decrease in NADPH was significantly (P < 0.05) attenuated by both the 0.3 and 3 mg/ml treatments of catechin. HG level significantly decreased NO compared with untreated cells. This increase in NO was significantly attenuated by the 0.3 mg/ml dose of the catechin.
In conclusion, catechin isolated from GMB-4 green tea prohibits the decrease in NADPH and NO in endothelial cells induced by HG. Therefore this may provide a natural therapy for attenuating the endothelial dysfunction found in diabetes mellitus.
本研究旨在调查从GMB - 4绿茶中分离出的儿茶素是否能够增加暴露于高糖（HG）水平的内皮细胞中的还原当量系统和一氧化氮（NO）水平。
从人脐血管组织中获取内皮细胞。汇合后，将人内皮细胞分为五组，包括对照组（未处理）、暴露于HG（30 mM）的内皮细胞、在以下三种剂量的绿茶儿茶素存在下暴露于HG的内皮细胞（HG + C）：0.03；0.3；和3 mg/ml。采用比色法分析NADP(+)、NADPH和NO水平。
0.3和3 mg/ml的儿茶素处理均显著（P < 0.05）减弱了NADPH的降低。与未处理的细胞相比，HG水平显著降低了NO。0.3 mg/ml剂量的儿茶素显著减弱了NO的增加。
总之，从GMB - 4绿茶中分离出的儿茶素可抑制HG诱导的内皮细胞中NADPH和NO的降低。因此，这可能为减轻糖尿病中发现的内皮功能障碍提供一种天然疗法。
