Copeland Anna Maria, Van Deusen Nicole M, Schmaljohn Connie S
United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.
United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.
Virology. 2015 Dec;486:88-93. doi: 10.1016/j.virol.2015.09.003. Epub 2015 Sep 25.
We investigated the localization of host mRNA during Rift Valley fever virus (RVFV) infection. Fluorescence in situ hybridization revealed that infection with RVFV altered the localization of host mRNA. mRNA accumulated in the nuclei of RVFV-infected but not mock-infected cells. Further, overexpression of the NSS gene, but not the N, GN or NSM genes correlated with mRNA nuclear accumulation. Nuclear accumulation of host mRNA was not observed in cells infected with a strain of RVFV lacking the gene encoding NSS, confirming that expression of NSS is likely responsible for this phenomenon.
我们研究了裂谷热病毒（RVFV）感染期间宿主mRNA的定位。荧光原位杂交显示，RVFV感染改变了宿主mRNA的定位。mRNA在RVFV感染的细胞而非模拟感染的细胞的细胞核中积累。此外，NSS基因的过表达而非N、GN或NSM基因的过表达与mRNA的核积累相关。在感染缺乏编码NSS基因的RVFV毒株的细胞中未观察到宿主mRNA的核积累，证实NSS的表达可能是造成这种现象的原因。
