Baker Kate S, Burnett Edward, McGregor Hannah, Deheer-Graham Ana, Boinett Christine, Langridge Gemma C, Wailan Alexander M, Cain Amy K, Thomson Nicholas R, Russell Julie E, Parkhill Julian
Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.
National Collection of Type Cultures, Public Health England, Porton Down, Salisbury, SP4 0JG, UK.
Genome Med. 2015 Sep 28;7:97. doi: 10.1186/s13073-015-0222-7.
Studies of historical isolates inform on the evolution and emergence of important pathogens and phenotypes, including antimicrobial resistance. Crucial to studying antimicrobial resistance are isolates that predate the widespread clinical use of antimicrobials. The Murray collection of several hundred bacterial strains of pre-antibiotic era Enterobacteriaceae is an invaluable resource of historical strains from important pathogen groups. Studies performed on the Collection to date merely exemplify its potential, which will only be realised through the continued effort of many scientific groups. To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains. Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content. We also present genomic analyses of population structure and determinants of mobilisable antimicrobial resistance to aid strain selection in future studies. This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.
对历史分离株的研究有助于了解重要病原体和表型的进化与出现，包括抗菌药物耐药性。对于研究抗菌药物耐药性而言，早于抗菌药物广泛临床使用的分离株至关重要。默里收集的数百株抗生素时代之前的肠杆菌科细菌菌株是来自重要病原体组的历史菌株的宝贵资源。迄今为止对该收集物进行的研究仅仅例证了其潜力，而这只有通过许多科学团队的持续努力才能实现。为实现这一目标，我们宣布默里收集物可通过国家典型培养物保藏中心公开获取，并呈现了超过半数菌株的全基因组序列数据及相关元数据。利用这些信息，我们核实了该收集物在亚组指定、等效分组和质粒含量方面的元数据。我们还展示了群体结构的基因组分析以及可移动抗菌药物耐药性的决定因素，以帮助未来研究中的菌株选择。这是研究这些重要病原体组以及抗菌药物耐药性的出现和进化的宝贵公共资源。
