Bafghi Ali Fatahi, Daghighi Mojtaba, Daliri Karim, Jebali Ali
Department of Medical Parasitology & Mycology, The School of Medicine, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Medical Nanotechnology, Pajoohesh Lab, Yazd, Iran.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:300-4. doi: 10.1016/j.colsurfb.2015.09.029. Epub 2015 Sep 21.
The aim of this study was to investigate the effect of magnesium oxide nanoparticles (MgO NPs) and MgO NPs coated with glucose (MONPCG) on Leishmania (L) major. First, the promastigotes of L. major were separately incubated with serial concentrations of MgO NPs and MONPCG for 24, 48, and 72 h at 37 °C. Then, the cell viability of promastigotes was evaluated by MTT assay. On the other hand, the relative expression of Cpb and GP63 genes was detected by quantitative-real time PCR. Based on results, the increase of concentration, both MgO NPs and MONPCG, and incubation time led to decrease of cell viability. Moreover, the expression of Cpb and GP63 genes was decreased with increase of concentration of MgO NPs and MONPCG. Also, the increase of incubation time led to decrease of their expression in MgO NPs treated promastogotes. But, in case of MONPCG treated promastogotes, the increase of incubation time did not change the expression of Cpb and GP63. Interestingly, MONPCG could silence Cpb and GP63 genes better than MgO NPs. Note, the capability was also seen at sub-toxic concentrations of MONPCG.
本研究旨在探讨氧化镁纳米颗粒（MgO NPs）和葡萄糖包被的氧化镁纳米颗粒（MONPCG）对硕大利什曼原虫（L）的影响。首先，将硕大利什曼原虫的前鞭毛体分别与系列浓度的MgO NPs和MONPCG在37℃下孵育24、48和72小时。然后，通过MTT法评估前鞭毛体的细胞活力。另一方面，通过定量实时PCR检测Cpb和GP63基因的相对表达。根据结果，MgO NPs和MONPCG浓度的增加以及孵育时间的延长均导致细胞活力下降。此外，Cpb和GP63基因的表达随着MgO NPs和MONPCG浓度的增加而降低。而且，孵育时间的延长导致MgO NPs处理的前鞭毛体中它们的表达下降。但是，在MONPCG处理的前鞭毛体中，孵育时间的延长并未改变Cpb和GP63的表达。有趣的是，MONPCG比MgO NPs能更好地沉默Cpb和GP63基因。注意，在MONPCG的亚毒性浓度下也观察到了这种能力。
