Rafiei Solmaz, Rezatofighi Seyedeh Elham, Ardakani Mohammad Roayaei, Madadgar Omid
Department of Biology, Faculty of Science, University of Shahid Chamran, Ahvaz, Iran.
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
IET Nanobiotechnol. 2015 Oct;9(5):247-51. doi: 10.1049/iet-nbt.2014.0028.
Foot-and-mouth disease (FMD) is an extremely contagious viral disease of cloven-hoofed animals that can lead to huge economic losses in the livestock production. No antiviral therapies are available for treating FMD virus (FMDV) infections in animals. The antiviral effects of magnesium oxide nanoparticles (MgO NPs) on the FMDV were investigated in cell culture. The viability of the cells after MgO NP treatment was determined using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The direct effects of MgO NPs on the FMDV in extracellular (virucidal assay) and also different stages of virus replication (antiviral assay) were evaluated by plaque reduction assay. The results showed that MgO NPs were safe at concentrations up to 250 µg/ml in the Razi Bovine kidney cell line. The treatments with NPs indicated that the MgO NPs exerted in vitro virucidal and antiviral activities. Plaque reduction assay revealed that MgO NPs can inhibit FMDV by more than 90% at the early stages of infection such as attachment and penetration but not after penetration. The results of this study suggested that NPs might be applied locally as an antiviral agent in early stages of infection in susceptible animals.
口蹄疫（FMD）是偶蹄目动物极易感染的病毒性疾病，可给畜牧生产造成巨大经济损失。目前尚无抗病毒疗法可用于治疗动物的口蹄疫病毒（FMDV）感染。在细胞培养中研究了氧化镁纳米颗粒（MgO NPs）对FMDV的抗病毒作用。使用MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐] 法测定MgO NP处理后细胞的活力。通过蚀斑减少试验评估MgO NPs对细胞外FMDV（杀病毒试验）以及病毒复制不同阶段（抗病毒试验）的直接作用。结果表明，在拉齐牛肾细胞系中，浓度高达250 µg/ml的MgO NPs是安全的。纳米颗粒处理表明，MgO NPs具有体外杀病毒和抗病毒活性。蚀斑减少试验表明，MgO NPs在感染早期如吸附和穿入阶段可抑制FMDV超过90%，但在穿入后则无此作用。本研究结果表明，纳米颗粒可在易感动物感染早期局部用作抗病毒剂。
