Whitelaw C Bruce A, Sheets Timothy P, Lillico Simon G, Telugu Bhanu P
The Roslin Institute and Royal (Dick) School of Veterinary Science, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Animal Bioscience and Biotechnology Laboratory, ARS, Beltsville, MD, 20705, USA.
J Pathol. 2016 Jan;238(2):247-56. doi: 10.1002/path.4648. Epub 2015 Nov 28.
The recent development of gene editing tools and methodology for use in livestock enables the production of new animal disease models. These tools facilitate site-specific mutation of the genome, allowing animals carrying known human disease mutations to be produced. In this review, we describe the various gene editing tools and how they can be used for a range of large animal models of diseases. This genomic technology is in its infancy but the expectation is that through the use of gene editing tools we will see a dramatic increase in animal model resources available for both the study of human disease and the translation of this knowledge into the clinic. Comparative pathology will be central to the productive use of these animal models and the successful translation of new therapeutic strategies.
用于家畜的基因编辑工具和方法的最新进展使得新型动物疾病模型的产生成为可能。这些工具有助于基因组的位点特异性突变，从而能够培育出携带已知人类疾病突变的动物。在本综述中，我们描述了各种基因编辑工具以及它们如何用于一系列大型动物疾病模型。这种基因组技术尚处于起步阶段，但预计通过使用基因编辑工具，我们将看到可用于人类疾病研究以及将这些知识转化到临床的动物模型资源大幅增加。比较病理学对于有效利用这些动物模型以及成功转化新的治疗策略至关重要。
