Bajpai Indu, Kim Duk Yeon, Kyong-Jin Jung, Song In-Hwan, Kim Sukyoung
School of Materials Science and Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeoungbuk, 712-749, South Korea.
Department of Anatomy, College of Medicine, Yeungnam University, 317-1 Daemyung-Dong, 705-717, South Korea.
J Biomed Mater Res B Appl Biomater. 2017 Jan;105(1):72-80. doi: 10.1002/jbm.b.33538. Epub 2015 Sep 28.
Calcium phosphates (Ca-P) are used commonly as artificial bone substitutes to control the biodegradation rate of an implant in the body fluid. This study examined the in vitro proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) on triphasic Ca-P samples. For this aspect, hydroxyapatite (HA), dicalcium phosphate dehydrate (DCPD), and calcium hydroxide (Ca(OH) ) were mixed at various ratios, cold compacted, and sintered at 1250°C in air. X-ray diffraction showed that the β-tricalcium phosphate (TCP) to α-TCP phase transformation increased with increasing DCPD/HA ratio. The micro-hardness deceased with increasing TCP content, whereas the mean grain size and porosity increased with increasing TCP concentration. To evaluate the in vitro degree of adhesion and proliferation on the HA/TCP samples, human BMSCs were incubated on the HA/TCP samples and analyzed by a cells proliferation assay, expression of the extracellular matrix (ECM) genes, such as α-smooth muscle actin (α-SMA) and fibronectin (FN), and FITC-phalloidin fluorescent staining. In terms of the interactions of human BMSCs with the triphasic Ca-P samples, H50T50 (Ca/P = 1.59) markedly enhanced cell spreading, proliferation, FN, and α-SMA compared with H100T0 (Ca/P = 1.67). Interestingly, these results show that among the five HA/TCP samples, H50T50 is the optimal Ca-P composition for in vitro cell proliferation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 72-80, 2017.
磷酸钙（Ca-P）通常用作人工骨替代物，以控制植入物在体液中的生物降解速率。本研究检测了人骨髓间充质干细胞（hBMSCs）在三相Ca-P样品上的体外增殖情况。在这方面，将羟基磷灰石（HA）、磷酸二钙二水合物（DCPD）和氢氧化钙（Ca(OH)₂）按不同比例混合，冷压成型，并在空气中1250°C烧结。X射线衍射表明，β-磷酸三钙（TCP）向α-TCP的相变随DCPD/HA比例的增加而增加。显微硬度随TCP含量的增加而降低，而平均晶粒尺寸和孔隙率随TCP浓度的增加而增加。为了评估在HA/TCP样品上的体外黏附程度和增殖情况，将人BMSCs接种在HA/TCP样品上，并通过细胞增殖测定、细胞外基质（ECM）基因（如α-平滑肌肌动蛋白（α-SMA）和纤连蛋白（FN））的表达以及FITC-鬼笔环肽荧光染色进行分析。就人BMSCs与三相Ca-P样品的相互作用而言，与H100T0（Ca/P = 1.67）相比，H50T50（Ca/P = 1.59）显著增强了细胞铺展、增殖、FN和α-SMA。有趣的是，这些结果表明，在五个HA/TCP样品中，H50T50是体外细胞增殖的最佳Ca-P组成。© 2015威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，105B：72 - 80，2017。
