Li Shiguo, Liu Chuang, Huang Jingliang, Liu Yangjia, Zheng Guilan, Xie Liping, Zhang Rongqing
Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
J Exp Biol. 2015 Nov;218(Pt 22):3623-31. doi: 10.1242/jeb.126748. Epub 2015 Sep 28.
Seawater acidification and warming resulting from anthropogenic production of carbon dioxide are increasing threats to marine ecosystems. Previous studies have documented the effects of either seawater acidification or warming on marine calcifiers; however, the combined effects of these stressors are poorly understood. In our study, we examined the interactive effects of elevated carbon dioxide partial pressure (P(CO2)) and temperature on biomineralization and amino acid content in an ecologically and economically important mussel, Mytilus edulis. Adult M. edulis were reared at different combinations of P(CO2) (pH 8.1 and 7.8) and temperature (19, 22 and 25°C) for 2 months. The results indicated that elevated P(CO2) significantly decreased the net calcification rate, the calcium content and the Ca/Mg ratio of the shells, induced the differential expression of biomineralization-related genes, modified shell ultrastructure and altered amino acid content, implying significant effects of seawater acidification on biomineralization and amino acid metabolism. Notably, elevated temperature enhanced the effects of seawater acidification on these parameters. The shell breaking force significantly decreased under elevated P(CO2), but the effect was not exacerbated by elevated temperature. The results suggest that the interactive effects of seawater acidification and elevated temperature on mussels are likely to have ecological and functional implications. This study is therefore helpful for better understanding the underlying effects of changing marine environments on mussels and other marine calcifiers.
人为产生的二氧化碳导致海水酸化和变暖，对海洋生态系统构成越来越大的威胁。以往的研究记录了海水酸化或变暖对海洋钙化生物的影响；然而，这些压力源的综合影响却知之甚少。在我们的研究中，我们研究了升高的二氧化碳分压（P(CO2)）和温度对一种在生态和经济上都很重要的贻贝——紫贻贝（Mytilus edulis）的生物矿化和氨基酸含量的交互作用。将成年紫贻贝在不同的P(CO2)（pH 8.1和7.8）和温度（19、22和25°C）组合下饲养2个月。结果表明，升高的P(CO2)显著降低了净钙化率、贝壳的钙含量和Ca/Mg比值，诱导了生物矿化相关基因的差异表达，改变了贝壳超微结构并改变了氨基酸含量，这意味着海水酸化对生物矿化和氨基酸代谢有显著影响。值得注意的是，升高温度增强了海水酸化对这些参数的影响。在升高的P(CO2)下，贝壳的断裂力显著降低，但升高温度并未加剧这种影响。结果表明，海水酸化和升高温度对贻贝的交互作用可能具有生态和功能意义。因此，这项研究有助于更好地理解不断变化的海洋环境对贻贝和其他海洋钙化生物的潜在影响。
