Ghazaryan Ara, Omar Murad, Tserevelakis George J, Ntziachristos Vasilis
Institute for Biological and Medical Imaging, Technische Universität München, Munich, Germany.
Institute for Biological and Medical Imaging, Technische Universität München, Munich, Germany ; Helmholtz Zentrum München, Munich, Germany.
Biomed Opt Express. 2015 Jul 31;6(9):3149-56. doi: 10.1364/BOE.6.003149. eCollection 2015 Sep 1.
Oxidative-based diseases including diabetes, chronic renal failure, cardiovascular diseases and neurological disorders are accompanied by accumulation of advanced glycation endproducts (AGE). Therefore, AGE-associated changes in tissue optical properties could yield a viable pathological indicator for disease diagnostics and monitoring. We investigated whether skin glycation could be detected based on absorption changes associated with AGE accumulation using spectral optoacoustic measurements and interrogated the optimal spectral band for skin glycation determination. Glycated and non-glycated skin was optoacoustically measured at multiple wavelengths in the visible region. The detected signals were spectrally processed and compared to measurements of skin auto-fluorescence and to second harmonic generation multiphoton microscopy images. Optoacoustic measurements are shown to be capable of detecting skin glycolysis based on AGE detection. A linear dependence was observed between optoacoustic intensity and the progression of skin glycation. The findings where corroborated by autofluorescence observations. Detection sensitivity is enhanced by observing normalised tissue spectra. This result points to a ratiometric method for skin glycation detection, specifically at 540 nm and 620 nm. We demonstrate that optoacoustic spectroscopy could be employed to detect AGE accumulation, and possibly can be employed as a non-invasive quick method for monitoring tissue glycation.
包括糖尿病、慢性肾衰竭、心血管疾病和神经紊乱在内的基于氧化的疾病都伴随着晚期糖基化终产物（AGE）的积累。因此，与AGE相关的组织光学特性变化可能为疾病诊断和监测提供一个可行的病理指标。我们研究了是否可以基于与AGE积累相关的吸收变化，利用光谱光声测量来检测皮肤糖基化，并探究用于确定皮肤糖基化的最佳光谱波段。在可见光区域的多个波长下对糖化皮肤和未糖化皮肤进行了光声测量。对检测到的信号进行光谱处理，并与皮肤自发荧光测量结果以及二次谐波产生多光子显微镜图像进行比较。结果表明，光声测量能够基于AGE检测来检测皮肤糖酵解。观察到光声强度与皮肤糖基化进程之间存在线性相关性。自发荧光观察结果证实了这些发现。通过观察归一化组织光谱提高了检测灵敏度。这一结果指向了一种用于皮肤糖基化检测的比率法，特别是在540纳米和620纳米处。我们证明光声光谱可用于检测AGE积累，并且可能作为一种监测组织糖基化的非侵入性快速方法。
