通过数字全息显微镜对三维血小板聚集体进行定量分析。
Quantitative analysis of platelets aggregates in 3D by digital holographic microscopy.
作者信息
Boudejltia Karim Zouaoui, Ribeiro de Sousa Daniel, Uzureau Pierrick, Yourassowsky Catherine, Perez-Morga David, Courbebaisse Guy, Chopard Bastien, Dubois Frank
机构信息
Laboratoire de Médecine Expérimentale (ULB 222 Unit), Medicine Faculty, Université libre de Bruxelles, CHU de Charleroi, Belgium.
Microgravity Research Centre, Université libre de Bruxelles, Brussels, Belgium.
出版信息
Biomed Opt Express. 2015 Aug 25;6(9):3556-63. doi: 10.1364/BOE.6.003556. eCollection 2015 Sep 1.
Abstract
Platelet spreading and retraction play a pivotal role in the platelet plugging and the thrombus formation. In routine laboratory, platelet function tests include exhaustive information about the role of the different receptors present at the platelet surface without information on the 3D structure of platelet aggregates. In this work, we develop, a method in Digital Holographic Microscopy (DHM) to characterize the platelet and aggregate 3D shapes using the quantitative phase contrast imaging. This novel method is suited to the study of platelets physiology in clinical practice as well as the development of new drugs.
摘要
血小板铺展和收缩在血小板黏附和血栓形成过程中起关键作用。在常规实验室中，血小板功能测试包含了血小板表面不同受体作用的详尽信息，但没有关于血小板聚集体三维结构的信息。在这项工作中，我们开发了一种数字全息显微镜（DHM）方法，利用定量相衬成像来表征血小板和聚集体的三维形状。这种新方法适用于临床实践中血小板生理学的研究以及新药的研发。
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