使用来自低成本数字全息显微镜的红细胞相位图像，通过一种新的相位函数测量糖化血红蛋白（HbA1c）。
Measurement of HbA1c with a new phase function using RBC phase image from a low-cost digital holographic microscopy.
作者信息
Sahu Ashok Kumar, Panda Bandita, Tripathy Adik Amiyan, Dash Sandip Kumar, Bal Atanu Kumar, Sahoo Naba Kishor, Tripathy Sukanta Kumar
机构信息
Department of Physics, Berhampur University, Ganjam, Berhampur, Odisha, 760007, India.
CoENSTds, Berhampur University, Ganjam, Odisha, 760007, India.
出版信息
Biotechnol Lett. 2025 Jun 23;47(4):65. doi: 10.1007/s10529-025-03610-7.
OBJECTIVE
Development of a new phase function for measuring HbA1c, using a low-cost digital holographic microscopy to monitor the glucose level in diabetes.
RESULTS
Here, we propose a new parameter, based on two different phases of RBCs, one, the absolute phase at the center, and the other, the average phase at the periphery, . The value varied almost linearly (R = 0.952) with HbA1c (%) in the blood. We used a low-cost digital holographic microscope (DHM) to record the hologram of RBCs, followed by digital reconstruction with the help of our newly introduced code. The critical value of at 3.5 was found to be equivalent to 6.5% HbA1c in high-performance liquid chromatography (HPLC), beyond which indicates hyperglycemia.
CONCLUSION
The method can be used for large-scale and low-cost estimation of HbA1c (%) with of ≥ 3.5.
目的
开发一种用于测量糖化血红蛋白（HbA1c）的新相位函数，采用低成本数字全息显微镜监测糖尿病患者的血糖水平。
结果
在此，我们基于红细胞的两个不同相位提出了一个新参数，一个是中心处的绝对相位，另一个是周边处的平均相位。该值与血液中糖化血红蛋白（HbA1c）（%）几乎呈线性变化（R = 0.952）。我们使用低成本数字全息显微镜（DHM）记录红细胞的全息图，然后借助我们新引入的代码进行数字重建。在高效液相色谱法（HPLC）中，发现该参数在3.5时的临界值相当于6.5%的糖化血红蛋白（HbA1c），超过此值表明血糖过高。
结论
该方法可用于大规模、低成本地估计糖化血红蛋白（HbA1c）（%），参数≥3.5 。
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