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柏科植物中SSR和EST-SSR位点的分离与鉴定

Isolation and characterization of SSR and EST-SSR loci in (Cupressaceae).

作者信息

Huang Chiun-Jr, Chu Fang-Hua, Liu Shau-Chian, Tseng Yu-Hsin, Huang Yi-Shiang, Ma Li-Ting, Wang Chieh-Ting, You Ya Ting, Hsu Shuo-Yu, Hsieh Hsiang-Chih, Chen Chi-Tsong, Chao Chi-Hsiang

机构信息

School of Forestry and Resource Conservation National Taiwan University No. 1, Sec. 4, Roosevelt Road Taipei 10617 Taiwan.

Department of Forensic Science Investigation Bureau Ministry of Justice No. 74, Zhonghua Road New Taipei City 23149 Taiwan.

出版信息

Appl Plant Sci. 2018 Aug 27;6(8):e01175. doi: 10.1002/aps3.1175. eCollection 2018 Aug.

DOI:10.1002/aps3.1175
PMID:30214838
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6110241/
Abstract

PREMISE OF THE STUDY

Simple sequence repeat (SSR) and expressed sequence tag (EST)-SSR markers were developed as tools for marker-assisted selection of and for the molecular differentiation of cypress species.

METHODS AND RESULTS

Based on the SSR-enriched genomic libraries and transcriptome data of , 300 primer pairs were selected for initial confirmation, of which 19 polymorphic SSR and eight polymorphic EST-SSR loci were chosen after testing in 92 individuals. The number of alleles observed for these 27 loci ranged from one to 17. The levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.000 to 0.903, respectively. Most markers also amplified in var. .

CONCLUSIONS

The developed SSR and EST-SSR sequences are the first reported markers specific to . These markers will be useful for individual identification of and to distinguish cypress species such as var. .

摘要

研究前提

开发简单序列重复（SSR）和表达序列标签（EST）-SSR标记，作为柏木属物种标记辅助选择和分子鉴别工具。

方法与结果

基于柏木属物种的SSR富集基因组文库和转录组数据，选择300对引物进行初步验证，经在92个个体中测试后，选取了19个多态性SSR和8个多态性EST-SSR位点。这27个位点观察到的等位基因数从1到17不等。观察到的杂合度水平和预期杂合度水平分别为0.000至1.000和0.000至0.903。大多数标记在柏木变种中也能扩增。

结论

开发的SSR和EST-SSR序列是首次报道的特定于柏木属物种的标记。这些标记将有助于柏木属物种的个体鉴定以及区分如柏木变种等柏木属物种。