Zaiko Anastasija, Samuiloviene Aurelija, Ardura Alba, Garcia-Vazquez Eva
Marine Science and Technology Centre, Klaipeda University, H. Manto 84, LT 92294 Klaipeda, Lithuania; Coastal and Freshwater Group, Cawthron Institute, 98 Halifax Street East, 7010 Nelson, New Zealand.
Marine Science and Technology Centre, Klaipeda University, H. Manto 84, LT 92294 Klaipeda, Lithuania.
Mar Pollut Bull. 2015 Nov 15;100(1):53-59. doi: 10.1016/j.marpolbul.2015.09.030. Epub 2015 Sep 28.
In this study, high-throughput sequencing (HTS) metabarcoding was applied for the surveillance of plankton communities within the southeastern (SE) Baltic Sea coastal zone. These results were compared with those from routine monitoring survey and morphological analyses. Four of five nonindigenous species found in the samples were identified exclusively by metabarcoding. All of them are considered as invasive in the Baltic Sea with reported impact on the ecosystem and biodiversity. This study indicates that, despite some current limitations, HTS metabarcoding can provide information on the presence of exotic species and advantageously complement conventional approaches, only requiring the same monitoring effort as before. Even in the currently immature status of HTS, this combination of HTS metabarcoding and observational records is recommended in the early detection of marine pests and delivery of the environmental status metrics of nonindigenous species.
在本研究中，高通量测序（HTS）元条形码技术被应用于波罗的海东南部（SE）沿海区域浮游生物群落的监测。这些结果与常规监测调查和形态分析的结果进行了比较。样本中发现的五个非本土物种中有四个仅通过元条形码技术得以识别。所有这些物种在波罗的海都被视为入侵物种，据报道它们对生态系统和生物多样性有影响。这项研究表明，尽管目前存在一些局限性，但HTS元条形码技术能够提供有关外来物种存在的信息，并能有效地补充传统方法，且只需与以往相同的监测工作量。即使在HTS目前尚不成熟的状态下，在早期检测海洋有害生物以及提供非本土物种的环境状况指标方面，仍建议将HTS元条形码技术与观测记录相结合。
