Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Slovakia.
Institute of Chemistry, Slovak Academy of Sciences, Slovakia.
Carbohydr Polym. 2015 Dec 10;134:516-23. doi: 10.1016/j.carbpol.2015.07.029. Epub 2015 Jul 19.
The ability to protect hyaluronic acid (HA) from oxidative degradation by cupric ions and ascorbate (production of (•)OH and peroxy-type radicals) during acute phase joint inflammation has been investigated using the following drugs: tiopronin, captopril, and levamisole. Radical scavenging activity, i.e. the propensity for donation of electrons was assessed for the drugs by ABTS and DPPH assays. The kinetics of HA degradation have been measured in the presence of each drug using rotational viscometry. The results of ABTS and DPPH assays show the highest radical scavenging activity for captopril, followed by tiopronin. For levamisole, no effect was observed. Captopril and tiopronin prevented HA degradation induced by (•)OH radicals in a similar manner, while tiopronin was more effective in scavenging peroxy-type radicals. On the other hand, levamisole was shown to be a pro-oxidant. Recovered HA fragments were characterized using FT-IR analysis, the incorporation of a sulphur atom from captopril and tiopronin but not from levamisole into the HA molecule was demonstrated.
我们研究了以下药物在急性关节炎症期间保护透明质酸（HA）免受铜离子和抗坏血酸（产生（•）OH 和过氧型自由基）氧化降解的能力：硫普罗宁、卡托普利和左旋咪唑。通过 ABTS 和 DPPH 测定法评估了药物的清除自由基活性，即电子捐赠倾向。使用旋转粘度计在每种药物存在的情况下测量了 HA 降解的动力学。ABTS 和 DPPH 测定的结果表明，卡托普利的清除自由基活性最高，其次是硫普罗宁。对于左旋咪唑，没有观察到效果。卡托普利和硫普罗宁以相似的方式阻止（•）OH 自由基诱导的 HA 降解，而硫普罗宁在清除过氧型自由基方面更有效。另一方面，左旋咪唑被证明是一种促氧化剂。使用傅里叶变换红外（FT-IR）分析对回收的 HA 片段进行了表征，证明了卡托普利和硫普罗宁而不是左旋咪唑的硫原子掺入 HA 分子中。
