Shah Kamal G, Guelig Dylan, Diesburg Steven, Buser Joshua, Burton Robert, LaBarre Paul, Richards-Kortum Rebecca, Weigl Bernhard
Department of Bioengineering, Rice University, Houston, Texas, United States of America; PATH, Seattle, Washington, United States of America.
PATH, Seattle, Washington, United States of America.
PLoS One. 2015 Oct 2;10(10):e0139449. doi: 10.1371/journal.pone.0139449. eCollection 2015.
Previous chemical heater designs for isothermal nucleic acid amplification have been based on solid-liquid phase transition, but using this approach, developers have identified design challenges en route to developing a low-cost, disposable device. Here, we demonstrate the feasibility of a new heater configuration suitable for isothermal amplification in which one reactant of an exothermic reaction is a liquid-gas phase-change material, thereby eliminating the need for a separate phase-change compartment. This design offers potentially enhanced performance and energy density compared to other chemical and electric heaters.
先前用于等温核酸扩增的化学加热器设计基于固液相转变，但采用这种方法时，开发者在开发低成本一次性设备的过程中发现了设计挑战。在此，我们展示了一种适用于等温扩增的新型加热器配置的可行性，其中放热反应的一种反应物是液-气相变材料，从而无需单独的相变隔室。与其他化学和电加热器相比，这种设计可能具有更高的性能和能量密度。
