Chen Jin-Yang, Mou Xiao-Zhou, Du Xiao-Chun, Xiang Charlie
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Clinical Research Institute, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
Asian Pac J Trop Med. 2015 Sep;8(9):739-46. doi: 10.1016/j.apjtm.2015.07.022. Epub 2015 Jul 29.
To invest the differences among mesenchymal stem cells (MSCs) derived from different tissues and their impacts on clinical applications.
In this study, MSCs were isolated from adipose tissue (AD), umbilical cord tissue (UC), and menstrual blood (Men) and compared their biological characteristics in terms of proliferation capacity, passage capacity, colony formation, and surface markers were compared.
The stem cells (SCs) obtained from different sources were all characterized as MSCs, but demonstrated some differences. Umbilical cord-derived MSCs (UCMSCs) were able to overcome density inhibition. The proliferation rate decreased in the order UCMSCs > MenSCs > ADSCs, while the colony-forming ability decreased in the order MenSCs > ADSCs > UCMSCs. Based on gene-expression data for MSCs from different sources within the same donor, 768 MenSC genes were found that were specifically upregulated or downregulated compared with bone marrow-derived MSCs and UCMSCs, most of which were involved in cell function-related pathways. In addition, MenSCs appeared to be superior in terms of immune inflammation, stress response, and neural differentiation potentials, but weaker in terms of osteogenic and chondrogenic differentiation capacities, compared with UCMSCs and bone marrow-derived MSCs.
MenSCs have higher extraction efficiency, colony-forming ability, and long time passage capacity. Although the proliferation capacity is inferior to UCMSCs.
研究不同组织来源的间充质干细胞（MSCs）之间的差异及其对临床应用的影响。
在本研究中，从脂肪组织（AD）、脐带组织（UC）和月经血（Men）中分离出MSCs，并比较它们在增殖能力、传代能力、集落形成和表面标志物方面的生物学特性。
从不同来源获得的干细胞（SCs）均被鉴定为MSCs，但存在一些差异。脐带源MSCs（UCMSCs）能够克服密度抑制。增殖率按UCMSCs＞MenSCs＞ADSCs的顺序降低，而集落形成能力按MenSCs＞ADSCs＞UCMSCs的顺序降低。基于同一供体内不同来源MSCs的基因表达数据，发现768个MenSC基因与骨髓源MSCs和UCMSCs相比有特异性上调或下调，其中大多数参与细胞功能相关途径。此外，与UCMSCs和骨髓源MSCs相比，MenSCs在免疫炎症、应激反应和神经分化潜能方面表现更优，但在成骨和成软骨分化能力方面较弱。
MenSCs具有更高的提取效率、集落形成能力和长时间传代能力。虽然增殖能力不如UCMSCs。
