Su Yu-Chang, Hsu Kuan-Ping, Hua Kuo-Feng, Ho Chen-Lung
Nat Prod Commun. 2015 Aug;10(8):1461-4.
The chemical composition and in vitro anti-inflammatory, antioxidant and antimicrobial activities of the essential oils isolated from the leaf and twig of Cupressus cashmeriana have been investigated. The essential oils were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The leaf oil mainly consisted of alpha-pinene (21.8%), epizonarene (8.0%), sabinene (7.9%), limonene (7.6%), gamma-terpinene (7.0%), and allo-aromadendrene (7.0%); the twig oil was mostly carvacrol methyl ether (35.4%), manool (16.1%), carvacrol (14.2%), and (2Z,6E)-farnesol (6.9%). Twig oil, but not leaf oil, was able to reduce nitric oxide production by lipopolysaccharide-activated murine macrophages RAW 264.7 without reducing the cell viability. In addition, twig oil showed better antioxidant and antimicrobial activities than leaf oil. The major ingredient of the twig oil that was responsible for the anti-inflammatory, antioxidant and antimicrobial activities was carvacrol.
对喜马拉雅柏树叶和嫩枝中分离得到的挥发油的化学成分及其体外抗炎、抗氧化和抗菌活性进行了研究。挥发油采用Clevenger型装置通过水蒸馏法分离，并分别用气相色谱-氢火焰离子化检测器（GC-FID）和气相色谱-质谱联用仪（GC-MS）进行表征。叶油主要由α-蒎烯（21.8%）、表紫穗槐烯（8.0%）、桧烯（7.9%）、柠檬烯（7.6%）、γ-松油烯（7.0%）和别香橙烯（7.0%）组成；嫩枝油主要成分是香芹酚甲醚（35.4%）、马尼醇（16.1%）、香芹酚（14.2%）和（2Z,6E）-法尼醇（6.9%）。嫩枝油而非叶油能够降低脂多糖激活的小鼠巨噬细胞RAW 264.7产生一氧化氮的量，且不降低细胞活力。此外，嫩枝油的抗氧化和抗菌活性优于叶油。嫩枝油中负责抗炎、抗氧化和抗菌活性的主要成分是香芹酚。
