Naz Iram, Mahmood Muhammad Khurram, Nagi Abdul Hannan
Oral Pathology, Islamabad Medical and Dental College, Islamabad, Pakistan E-mail :
Asian Pac J Cancer Prev. 2015;16(15):6289-92. doi: 10.7314/apjcp.2015.16.15.6289.
To determine the biological behaviour of common odontogenic cystic lesions by analysing and comparing bcl-2 expression amongst them.
Our study covered 90 formalin fixed paraffin embedded tissue samples: 26 primary cases each of radicular cysts (RC), dentigerous cysts (DC) and odontogenic keratocysts (OKC) and 12 of recurrent OKCs. Bcl-2 expression was analysed immunohistochemically and data analysis was accomplished using SPSS version 17.0. Means were taken for age while for gender and site of the lesions frequencies and percentages were determined. The Chi-square test was applied to evaluate any statistically significant difference of bcl-2 expression in these lesions and p value of ≤0.05 was taken as significant.
All the recurrent OKCs showed a strong positivity for bcl-2 that was absent in all of its primary cases (p value<0.05). Although variation in expression of bcl-2 was not found to be statistically significant between RC and DC, however, it became significant when all primary cases of these common odontogenic lesions were compared.
Recurrent OKC showed comparatively a more aggressive behaviour than their primary counterparts and also from RC and DC. Bcl-2 proved to be a valuable adjunct in determining aggressive biological behaviour of odontogenic lesions.
通过分析和比较常见牙源性囊性病变中bcl-2的表达，确定其生物学行为。
我们的研究涵盖90个福尔马林固定石蜡包埋组织样本：26例原发性根囊肿（RC）、含牙囊肿（DC）和牙源性角化囊肿（OKC），以及12例复发性OKC。采用免疫组织化学方法分析bcl-2表达，并使用SPSS 17.0版进行数据分析。计算年龄均值，确定病变的性别、部位的频率和百分比。应用卡方检验评估这些病变中bcl-2表达的统计学显著差异，p值≤0.05被视为有显著性差异。
所有复发性OKC均显示bcl-2强阳性，而其所有原发性病例均未出现bcl-2阳性（p值<0.05）。虽然未发现RC和DC之间bcl-2表达的差异具有统计学意义，但是，当比较这些常见牙源性病变的所有原发性病例时，差异具有统计学意义。
复发性OKC比其原发性病变以及RC和DC表现出更具侵袭性的行为。bcl-2被证明是确定牙源性病变侵袭性生物学行为的有价值辅助指标。
