根端囊肿和牙源性角化囊肿细胞因子产生的分析表明存在共同的生长机制。

Profiling of radicular cyst and odontogenic keratocyst cytokine production suggests common growth mechanisms.

作者信息

Hayashi Makoto, Ohshima Takafumi, Ohshima Mitsuhiro, Yamaguchi Yoko, Miyata Hirofumi, Takeichi Osamu, Ogiso Bunnai, Ito Koichi, Ostman Arne, Otsuka Kichibee

机构信息

Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan.

出版信息

J Endod. 2008 Jan;34(1):14-21. doi: 10.1016/j.joen.2007.08.020. Epub 2007 Nov 26.

DOI:10.1016/j.joen.2007.08.020

PMID:18155485

Abstract

The aim of this study was to compare the cytokine expression profiles of cyst fluids (CFs) and tissue culture supernatants (SUPs) from 7 radicular cysts (RCs) and 7 odontogenic keratocysts (OKCs) by using Human Cytokine Antibody Array to identify the specific cytokines involved in formation and expansion of RCs and OKCs, respectively. There were significant differences in relative expression levels of IL-1 beta, MCP1, MIP1 beta, FGF-9, GDNF, HGF, IGFBP-3, Ang, IP-10, MIF, OPG, and TGF-beta2 between RC-CF and OKC-CF (P < .05). On the other hand, the cytokine expression patterns of RC-SUP (HGF, IL-8, NAP-2, IL-6, TIMP-1 and 2, GRO, IP-10, and Ang) were similar to those of OKC-SUP. Only the relative expression level of GRO differed between RC-SUP and OKC-SUP (P < .05). The similarities of cytokine production by tissue cultures derived from RC and OKC indicate that the expansion mechanisms of RC and OKC might involve similar biologic mechanisms other than infection.

摘要

本研究旨在通过使用人细胞因子抗体阵列比较7个根尖囊肿（RCs）和7个牙源性角化囊肿（OKCs）的囊液（CFs）和组织培养上清液（SUPs）中的细胞因子表达谱，以分别鉴定参与RCs和OKCs形成与扩大的特定细胞因子。RC - CF和OKC - CF之间IL - 1β、MCP1、MIP1β、FGF - 9、GDNF、HGF、IGFBP - 3、Ang、IP - 10、MIF、OPG和TGF - β2的相对表达水平存在显著差异（P < 0.05）。另一方面，RC - SUP（HGF、IL - 8、NAP - 2、IL - 6、TIMP - 1和2、GRO、IP - 10和Ang）的细胞因子表达模式与OKC - SUP相似。RC - SUP和OKC - SUP之间仅GRO的相对表达水平存在差异（P < 0.05）。来自RC和OKC的组织培养物产生细胞因子的相似性表明，RC和OKC的扩大机制可能涉及除感染之外的相似生物学机制。