College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
J Med Virol. 2019 Sep;91(9):1595-1601. doi: 10.1002/jmv.25494. Epub 2019 May 29.
Foot-and-mouth disease (FMD) is a highly contagious disease that affects cloven-hoof animals including cattle, swine, sheep, goats, and lots of wild species. Effectively control measures are urged needed. Here, we showed that homoharringtonine treatment exhibited a strong inhibitory effect against two different strains of FMDVs (O/MYA98/BY/2010 and A/GD/MM/2013) in swine kidney (IBRS-2) cells. Further experiments demonstrated that homoharringtonine did not affect virus attachment or entry. Using time-of-addition assays, we found that the antiviral activity of homoharringtonine occurred primarily during the early stage of infection. These results demonstrated that homoharringtonine might be an effective anti-FMDV drug. Further studies are required to explore the antiviral activity of homoharringtonine against FMDV replication in vivo.
口蹄疫（FMD）是一种高度传染性疾病，影响偶蹄动物，包括牛、猪、绵羊、山羊和许多野生动物。需要采取有效的控制措施。在这里，我们表明，高三尖杉酯碱处理对猪肾（IBRS-2）细胞中的两种不同的 FMDV（O/MYA98/BY/2010 和 A/GD/MM/2013）表现出强烈的抑制作用。进一步的实验表明，高三尖杉酯碱不影响病毒附着或进入。通过时间加药实验，我们发现高三尖杉酯碱的抗病毒活性主要发生在感染的早期阶段。这些结果表明，高三尖杉酯碱可能是一种有效的抗 FMDV 药物。需要进一步研究来探索高三尖杉酯碱在体内抗 FMDV 复制的抗病毒活性。
