Yang L, Chen S J, Yuan G Y, Wang D, Chen J J
Department of Endocrinology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China.
Genet Mol Res. 2015 Sep 25;14(3):11356-61. doi: 10.4238/2015.September.25.2.
We investigated serum visceral adipose tissue-derived serpin (vaspin) levels in patients with normal glucose regulation and recently diagnosed type 2 diabetes (T2DM) and explored the association between vaspin and body mass index, age, gender, glucose, lipid metabolism, and insulin sensitivity. Fasting serum vaspin levels in 66 patients with T2DM and 48 normal subjects were detected using enzyme-linked immunosorbent assay. We found that serum vaspin levels in the DM group were 0.65 ± 0.13 mg/L in non-obese patients and 1.13 ± 0.25 mg/L in obese patients. Serum vaspin levels in the control group were 0.38 ± 0.18 mg/L in non-obese patients and 0.95 ± 0.11 mg/L in obese patients. Average serum vaspin levels were significantly higher in obese patients than in non-obese patients in both the DM group and control group. In the DM group, the serum vaspin level was 0.76 ± 0.22 mg/L in males and 0.92 ± 0.35 mg/L in females. In the control group, the serum vaspin level was 0.48 ± 0.14 mg/L in males and 1.05 ± 0.21 mg/L in females. Association analysis showed that serum vaspin levels were significantly associated with body mass index, waist-to-rip ratio (WHR), fat percentage, triglyceride, fasting plasma insulin, and insulin sensitivity index. Stepwise multiple regression analysis showed that gender, insulin sensitivity index, and WHR were the most significant independent factors affecting vaspin. Therefore, serum vaspin levels were significantly elevated in obese people and were independently associated with WHR, gender, and index sensitivity index.
我们研究了血糖调节正常的患者以及近期诊断为2型糖尿病（T2DM）患者的血清内脏脂肪组织衍生丝氨酸蛋白酶抑制剂（vaspin）水平，并探讨了vaspin与体重指数、年龄、性别、血糖、脂质代谢及胰岛素敏感性之间的关联。采用酶联免疫吸附测定法检测了66例T2DM患者和48例正常受试者的空腹血清vaspin水平。我们发现，糖尿病组非肥胖患者的血清vaspin水平为0.65±0.13mg/L，肥胖患者为1.13±0.25mg/L。对照组非肥胖患者的血清vaspin水平为0.38±0.18mg/L，肥胖患者为0.95±0.11mg/L。糖尿病组和对照组中，肥胖患者的平均血清vaspin水平均显著高于非肥胖患者。在糖尿病组中，男性血清vaspin水平为0.76±0.22mg/L，女性为0.92±0.35mg/L。在对照组中，男性血清vaspin水平为0.48±0.14mg/L，女性为1.05±0.21mg/L。相关性分析显示，血清vaspin水平与体重指数、腰臀比（WHR）、脂肪百分比、甘油三酯、空腹血浆胰岛素及胰岛素敏感性指数显著相关。逐步多元回归分析显示，性别、胰岛素敏感性指数和WHR是影响vaspin的最显著独立因素。因此，肥胖人群的血清vaspin水平显著升高，且与WHR、性别及胰岛素敏感性指数独立相关。
