脉冲电磁场疗法对骨髓间充质干细胞成骨及成脂分化的影响
Effect of pulsed electromagnetic field therapy on the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells.
作者信息
Lu T, Huang Y X, Zhang C, Chai M X, Zhang J
机构信息
Second Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
First Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
出版信息
Genet Mol Res. 2015 Sep 28;14(3):11535-42. doi: 10.4238/2015.September.28.5.
We investigated the effects of pulsed electromagnetic fields (PEMFs) of 20 Hz/2 mT on the osteogenic and adipogenic differentiation of bone marrow stem cells (BMSCs). Sprague Dawley rat BMSCs were isolated and cultured in vitro. The BMSCs of the third passage were obtained and stimulated by PEMFs of 20 Hz/2 mT. The alkaline phosphatase (ALP) activity was measured according to the ALP assay kit manufacturer instructions, the BMSC osteogenic and adipogenic indicators were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and oil red O staining was used to observe the adipose-induced adipogenic differentiation of BMSCs. PEMFs of 20 Hz/2 mT significantly promoted the activity of ALP in the BMSCs (P < 0.01) and mRNA expression of osteogenic proteins (osteocalcin and osteopontin). The PEMFs inhibited the expression of adipogenic transcription factors such as adipokines and adipocyte-binding protein-2, and the adipogenic differentiation of BMSCs. PEMFs of 20 Hz/2 mT can promote osteogenic differentiation and inhibit adipogenic differentiation in BMSCs.
我们研究了20 Hz/2 mT的脉冲电磁场（PEMFs）对骨髓干细胞（BMSCs）成骨和成脂分化的影响。分离Sprague Dawley大鼠的BMSCs并进行体外培养。获取第三代BMSCs并用20 Hz/2 mT的PEMFs进行刺激。根据碱性磷酸酶（ALP）检测试剂盒制造商的说明测量ALP活性，通过半定量逆转录聚合酶链反应（RT-PCR）检测BMSCs的成骨和成脂指标，并用油红O染色观察BMSCs的脂肪诱导成脂分化。20 Hz/2 mT的PEMFs显著促进了BMSCs中ALP的活性（P < 0.01）以及成骨蛋白（骨钙素和骨桥蛋白）的mRNA表达。PEMFs抑制了脂肪因子和脂肪细胞结合蛋白-2等脂肪生成转录因子的表达以及BMSCs的成脂分化。20 Hz/2 mT的PEMFs可促进BMSCs的成骨分化并抑制其成脂分化。
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