使用定制照明和成像模块改装的手术显微镜对ABY-029进行微剂量荧光成像。
Microdose fluorescence imaging of ABY-029 on an operating microscope adapted by custom illumination and imaging modules.
作者信息
Elliott Jonathan T, Dsouza Alisha V, Marra Kayla, Pogue Brian W, Roberts David W, Paulsen Keith D
机构信息
Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA.
Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA; Department of Surgery, Geisel School of Medicine at Dartmouth, 1 Rope Ferry Road, Hanover, NH 03755, USA.
出版信息
Biomed Opt Express. 2016 Aug 9;7(9):3280-3288. doi: 10.1364/BOE.7.003280. eCollection 2016 Sep 1.
Abstract
Fluorescence guided surgery has the potential to positively impact surgical oncology; current operating microscopes and stand-alone imaging systems are too insensitive or too cumbersome to maximally take advantage of new tumor-specific agents developed through the microdose pathway. To this end, a custom-built illumination and imaging module enabling picomolar-sensitive near-infrared fluorescence imaging on a commercial operating microscope is described. The limits of detection and system specifications are characterized, and efficacy of the system in detecting ABY-029 is evaluated in a rat orthotopic glioma model following microdose injections, showing the suitability of the device for microdose phase 0 clinical trials.
摘要
荧光引导手术有潜力对手术肿瘤学产生积极影响；目前的手术显微镜和独立成像系统过于不灵敏或过于笨重，无法充分利用通过微剂量途径开发的新型肿瘤特异性药物。为此，本文描述了一种定制的照明和成像模块，该模块可在商用手术显微镜上实现皮摩尔敏感的近红外荧光成像。对该系统的检测限和系统规格进行了表征，并在微剂量注射后的大鼠原位胶质瘤模型中评估了该系统检测ABY-029的效果，结果表明该设备适用于微剂量0期临床试验。
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本文引用的文献
1
Simultaneous Fluorescent Markers for Perfusion, Protoporphyrin Metabolism, and EGFR Expression for Optically Guided Identification of Orthotopic Glioma.用于原位胶质瘤光学引导识别的灌注、原卟啉代谢和表皮生长因子受体表达的同步荧光标记物
Clin Cancer Res. 2017 May 1;23(9):2203-2212. doi: 10.1158/1078-0432.CCR-16-1400. Epub 2016 Oct 31.
2
Review of fluorescence guided surgery systems: identification of key performance capabilities beyond indocyanine green imaging.荧光引导手术系统的评价：超越吲哚菁绿成像的关键性能能力的识别。
J Biomed Opt. 2016 Aug 1;21(8):80901. doi: 10.1117/1.JBO.21.8.080901.
3
Review of fluorescence guided surgery visualization and overlay techniques.荧光引导手术可视化与叠加技术综述。
Biomed Opt Express. 2015 Sep 3;6(10):3765-82. doi: 10.1364/BOE.6.003765. eCollection 2015 Oct 1.
4
Augmented microscopy: real-time overlay of bright-field and near-infrared fluorescence images.增强显微镜：明场和近红外荧光图像的实时叠加
J Biomed Opt. 2015 Oct;20(10):106002. doi: 10.1117/1.JBO.20.10.106002.
5
Fluorescence-guided surgery with live molecular navigation--a new cutting edge.荧光引导手术与实时分子导航——新的前沿技术。
Nat Rev Cancer. 2013 Sep;13(9):653-62. doi: 10.1038/nrc3566. Epub 2013 Aug 8.
6
Image-guided cancer surgery using near-infrared fluorescence.近红外荧光引导的癌症手术。
Nat Rev Clin Oncol. 2013 Sep;10(9):507-18. doi: 10.1038/nrclinonc.2013.123. Epub 2013 Jul 23.
7
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Nat Med. 2011 Sep 18;17(10):1315-9. doi: 10.1038/nm.2472.
9
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J Surg Oncol. 2011 Sep 1;104(3):323-32. doi: 10.1002/jso.21943. Epub 2011 Apr 14.
10
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