Elliott Jonathan T, Dsouza Alisha V, Marra Kayla, Pogue Brian W, Roberts David W, Paulsen Keith D
Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA.
Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA; Department of Surgery, Geisel School of Medicine at Dartmouth, 1 Rope Ferry Road, Hanover, NH 03755, USA.
Biomed Opt Express. 2016 Aug 9;7(9):3280-3288. doi: 10.1364/BOE.7.003280. eCollection 2016 Sep 1.
Fluorescence guided surgery has the potential to positively impact surgical oncology; current operating microscopes and stand-alone imaging systems are too insensitive or too cumbersome to maximally take advantage of new tumor-specific agents developed through the microdose pathway. To this end, a custom-built illumination and imaging module enabling picomolar-sensitive near-infrared fluorescence imaging on a commercial operating microscope is described. The limits of detection and system specifications are characterized, and efficacy of the system in detecting ABY-029 is evaluated in a rat orthotopic glioma model following microdose injections, showing the suitability of the device for microdose phase 0 clinical trials.
荧光引导手术有潜力对手术肿瘤学产生积极影响；目前的手术显微镜和独立成像系统过于不灵敏或过于笨重，无法充分利用通过微剂量途径开发的新型肿瘤特异性药物。为此，本文描述了一种定制的照明和成像模块，该模块可在商用手术显微镜上实现皮摩尔敏感的近红外荧光成像。对该系统的检测限和系统规格进行了表征，并在微剂量注射后的大鼠原位胶质瘤模型中评估了该系统检测ABY-029的效果，结果表明该设备适用于微剂量0期临床试验。
