Weinstock Ada, Fisher Edward A
Departments of Medicine (Cardiology) and Cell Biology, and the Marc and Ruti Bell Program in Vascular Biology, New York University School of Medicine, New York, NY, USA.
Methods Mol Biol. 2019;1951:153-165. doi: 10.1007/978-1-4939-9130-3_12.
Monocytes are circulating cells imperative to the response against pathogens. Upon infection, they are quickly recruited to the affected tissue where they can differentiate into specialized phagocytes and antigen-presenting cells. Additionally, monocytes play a vital role in chronic inflammation, where they can promote and enhance inflammation or induce its resolution. There are two major subsets of monocytes, "inflammatory" and "nonclassical," which are believed to have distinct functions. In atherosclerosis, both types of monocytes are constantly recruited to lesions, where they contribute to plaque formation and atherosclerosis progression. Surprisingly, these cells can also be recruited to lesions and promote resolution of atherosclerosis. Tracking these cells in various disease stages may inform about the dynamic changes occurring in the inflamed and resolving tissues. In this chapter we will discuss methods for differential labeling of the two monocyte subsets in order to examine their dynamics in inflammation.
单核细胞是循环细胞，对抵抗病原体的反应至关重要。感染后，它们会迅速被招募到受影响的组织，在那里它们可以分化为特殊的吞噬细胞和抗原呈递细胞。此外，单核细胞在慢性炎症中起着至关重要的作用，它们可以促进和增强炎症或诱导炎症消退。单核细胞有两个主要亚群，即“炎症性”和“非经典性”，据信它们具有不同的功能。在动脉粥样硬化中，这两种类型的单核细胞都会不断被招募到病变部位，在那里它们促进斑块形成和动脉粥样硬化进展。令人惊讶的是，这些细胞也可以被招募到病变部位并促进动脉粥样硬化的消退。在不同疾病阶段追踪这些细胞可能会了解炎症组织和正在消退的组织中发生的动态变化。在本章中，我们将讨论对两个单核细胞亚群进行差异标记的方法，以便研究它们在炎症中的动态变化。
