Shahin Kifah, Mahmoudifar Nastaran, Doran Pauline M
Westmead Millennium Institute for Medical Research, University of Sydney, Westmead, NSW, 2145, Australia.
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Methods Mol Biol. 2015;1340:25-40. doi: 10.1007/978-1-4939-2938-2_2.
As the only cell type found in healthy adult cartilage, chondrocytes are the obvious and most direct starting point for cartilage tissue engineering. Human adult, juvenile, neonatal, and fetal chondrocytes have all been demonstrated to produce cartilage matrix components in vitro for production of engineered tissues. In this chapter, procedures are outlined for isolation of chondrocytes from human fetal and adult cartilage. Methods for expansion and cryopreservation of the cells and characterization of gene expression using quantitative polymerase chain reaction (Q-PCR) analysis are also described.
作为健康成人软骨中唯一发现的细胞类型，软骨细胞是软骨组织工程明显且最直接的起点。人类成人、青少年、新生儿和胎儿软骨细胞均已被证明在体外能够产生软骨基质成分以用于构建工程组织。在本章中，概述了从人类胎儿和成人软骨中分离软骨细胞的步骤。还描述了细胞的扩增、冷冻保存方法以及使用定量聚合酶链反应（Q-PCR）分析进行基因表达表征的方法。
