Ostrowski M, Minden M, Wang C, Bailey D
Department of Pathology, Toronto General Hospital, Ontario, Canada.
Am J Clin Pathol. 1989 Feb;91(2):215-21. doi: 10.1093/ajcp/91.2.215.
A rare case of diffuse large cell lymphoma (DLL) arising in the stomach of a patient with long-standing chronic lymphocytic leukemia (CLL) has been examined by a combination of immunophenotypic and gene probe methods. Both the resected lymphoma and the CLL (obtained from an infiltrate in perigastric lymph nodes) expressed surface membrane markers consistent with a B-cell origin (HLA-DR+; B1+; B4+), but surface membrane immunoglobulin (SmIg) could be detected only on the CLL cells (SmIgM lambda+). Neither neoplasm expressed cytoplasmic immunoglobulin. DNA probe analysis using the Southern blot method and probes to the joining (J-H) region of the immunoglobulin heavy chain gene and to the constant (C) regions of the immunoglobulin kappa and lambda light chain genes revealed different light and heavy chain gene rearrangements in the two neoplasms. These findings suggest that the large cell lymphoma arose from a different clone than the CLL and the development of large cell lymphoma may have represented a de novo neoplastic event in a predisposed person.
采用免疫表型和基因探针方法相结合，对1例长期患有慢性淋巴细胞白血病（CLL）的患者胃内发生的罕见弥漫大细胞淋巴瘤（DLL）进行了检查。切除的淋巴瘤和CLL（取自胃周淋巴结浸润处）均表达与B细胞起源一致的表面膜标志物（HLA-DR+；B1+；B4+），但仅在CLL细胞上可检测到表面膜免疫球蛋白（SmIg）（SmIgM λ+）。两种肿瘤均未表达细胞质免疫球蛋白。使用Southern印迹法及免疫球蛋白重链基因连接（J-H）区探针和免疫球蛋白κ及λ轻链基因恒定（C）区探针进行的DNA探针分析显示，两种肿瘤中轻链和重链基因重排不同。这些发现提示，大细胞淋巴瘤起源于与CLL不同的克隆，大细胞淋巴瘤的发生可能代表了易感个体中的一种新发肿瘤事件。
