Titilawo Yinka, Obi Larry, Okoh Anthony
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, 5700, South Africa.
Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice, 5700, South Africa.
BMC Microbiol. 2015 Oct 8;15:204. doi: 10.1186/s12866-015-0540-3.
Diarrhoeal diseases are attributable to unsafe water stemming from improper sanitation and hygiene and are reportedly responsible for extensive morbidity and mortality particularly among children in developed and developing countries.
Water samples from selected rivers in Osun State, South-Western Nigeria were collected and analyzed using standard procedures. Escherichia coli isolates (n=300) were screened for 10 virulence genes using polymerase chain reaction for pathotyping.
While the virulence gene (VG) lt for enterotoxigenic E. coli had the highest prevalence of 45%, the enteropathogenic E. coli genes eae and bfp were detected in 6 and 4% of the isolates respectively. The VGs stx1 and stx2 specific for the enterohemorrhagic E. coli pathotypes were detected in 7 and 1% of the isolates respectively. Also, the VG eagg harboured by enteroaggregative pathotype and diffusely-adherent E. coli VG daaE were detected in 2 and 4% of the isolates respectively and enteroinvasive E. coli VG ipaH was not detected. In addition, the VGs papC for uropathogenic and ibeA for neonatal meningitis were frequently detected in 19 and 3% of isolates respectively.
These findings reveal the presence of diarrhoeagenic and non-diarrhoeagenic E. coli in the selected rivers and a potential public health risk as the rivers are important resources for domestic, recreational and livelihood usage by their host communities.
腹泻病可归因于因卫生设施和卫生习惯不当导致的不安全用水，据报道，腹泻病会导致大量发病和死亡，尤其是在发达国家和发展中国家的儿童中。
采集尼日利亚西南部奥孙州选定河流的水样，并使用标准程序进行分析。使用聚合酶链反应对300株大肠杆菌分离株进行10种毒力基因筛查，以进行致病型分类。
产肠毒素大肠杆菌的毒力基因lt的流行率最高，为45%，而肠致病性大肠杆菌基因eae和bfp分别在6%和4%的分离株中被检测到。分别在7%和1%的分离株中检测到了针对肠出血性大肠杆菌致病型的毒力基因stx1和stx2。此外，分别在2%和4%的分离株中检测到了肠聚集性致病型携带的毒力基因eagg和弥漫性黏附大肠杆菌毒力基因daaE，未检测到肠侵袭性大肠杆菌毒力基因ipaH。此外，分别在19%和3%的分离株中频繁检测到了尿路致病性毒力基因papC和新生儿脑膜炎毒力基因ibeA。
这些发现揭示了所选河流中存在致腹泻性和非致腹泻性大肠杆菌，并且存在潜在的公共卫生风险，因为这些河流是其所在社区重要的生活、娱乐和生计用水资源。
