N-烷酰胺对长时间游泳小鼠耐力和抗疲劳性能的影响。

Effects of macamides on endurance capacity and anti-fatigue property in prolonged swimming mice.

作者信息

Yang Qin, Jin Wenwen, Lv Xueyuan, Dai Pengfei, Ao Yanxiao, Wu Mengying, Deng Wenjing, Yu Longjiang

机构信息

a Department of Biotechnology, Institute of Resource Biology and Biotechnology , College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , China .

b School of Physical Education and Sport , Central China Normal University , Wuhan , China .

出版信息

Pharm Biol. 2016;54(5):827-34. doi: 10.3109/13880209.2015.1087036. Epub 2015 Oct 9.

DOI:10.3109/13880209.2015.1087036

PMID:26453017

Abstract

CONTEXT

Lepidium meyenii Walp. (Brassicaceae), most commonly known as "maca", has been used as a food or folk medicine to improve vitality in Peru. Previous research demonstrated that lipid-soluble extract from maca improved swimming endurance capacity. Macamides are considered the typical lipid-soluble markers for maca and proved to have several pharmacological properties, such as improving sexual performance and neuroprotective activies.

OBJECTIVE

The present study investigates the effects of macamides on endurance capacity and anti-fatigue property in prolonged swimming mice.

MATERIALS AND METHODS

The Balb/c mice were divided into seven groups: a control group, low-dose groups of N-benzyllinoleamide, N-benzyloleamide, and N-benzylpalmitamide, high-dose groups of these macamides. The macamides groups received the commercial products (12 and 40 mg/kg, ig), while the control group received vehicle for 21 d. On the 14th day, the mice were given the weight-loaded swimming test. On the 21st day, the mice were sacrificed immediately after 90 min swimming, and some biochemical parameters were measured.

RESULTS AND DISCUSSION

Compared with the control group, exhaustive swimming time was significantly prolonged in high-dose group of N-benzyloleamide (p < 0.05); the levels of lactic acid (LD), blood ammonia (BA), and lactate dehydrogenase (LDH) were significantly decreased (p < 0.05), whereas the levels of liver glycogen (LG) and non-esterified fatty acid (NEFA) were significantly increased (p < 0.05) in high-dose group of N-benzyloleamide. The malondialdehyde (MDA) contents in the brain, muscle, and liver were significantly decreased (p < 0.05), whereas superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities in the brain, muscle, and liver were significantly increased in high-dose group of N-benzyloleamide (p < 0.05).

CONCLUSION

The results indicate that N-benzyloleamide has pharmaceutical property against exercise-induced fatigue, and this effect can be explained by the modulated energy metabolism and improved antioxidant status.

摘要

背景

玛咖（Lepidium meyenii Walp.，十字花科），最为人熟知的名称是“玛卡”，在秘鲁一直被用作食物或民间药物来增强活力。先前的研究表明，玛卡的脂溶性提取物可提高游泳耐力。玛卡酰胺被认为是玛卡的典型脂溶性标志物，并已证明具有多种药理特性，如改善性功能和神经保护活性。

目的

本研究探讨玛卡酰胺对长时间游泳小鼠耐力和抗疲劳性能的影响。

材料与方法

将Balb/c小鼠分为七组：对照组、N-苄基亚油酸酰胺低剂量组、N-苄基油酸酰胺低剂量组、N-苄基棕榈酰胺低剂量组、这些玛卡酰胺的高剂量组。玛卡酰胺组给予商业产品（12和40mg/kg，灌胃），而对照组给予赋形剂，持续21天。在第14天，对小鼠进行负重游泳试验。在第21天，小鼠在游泳90分钟后立即处死，并测量一些生化参数。

结果与讨论

与对照组相比，N-苄基油酸酰胺高剂量组的力竭游泳时间显著延长（p<0.05）；高剂量N-苄基油酸酰胺组的乳酸（LD）、血氨（BA）和乳酸脱氢酶（LDH）水平显著降低（p<0.05），而肝糖原（LG）和非酯化脂肪酸（NEFA）水平显著升高（p<0.05）。高剂量N-苄基油酸酰胺组脑、肌肉和肝脏中的丙二醛（MDA）含量显著降低（p<0.05），而脑、肌肉和肝脏中的超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）活性显著升高（p<0.05）。