DeNardo Laura A, Berns Dominic S, DeLoach Katherine, Luo Liqun
Howard Hughes Medical Institute and Department of Biology, Stanford University, Stanford, CA 94305.
Neurosciences Program, Stanford University, Stanford, CA 94305.
Nat Neurosci. 2015 Nov;18(11):1687-1697. doi: 10.1038/nn.4131. Epub 2015 Oct 12.
Information processing in neocortical circuits requires integrating inputs over a wide range of spatial scales, from local microcircuits to long-range cortical and subcortical connections. We used rabies virus-based trans-synaptic tracing to analyze the laminar distribution of local and long-range inputs to pyramidal neurons in the mouse barrel cortex and medial prefrontal cortex (mPFC). In barrel cortex, we found substantial inputs from layer 3 (L3) to L6, prevalent translaminar inhibitory inputs, and long-range inputs to L2/3 or L5/6 preferentially from L2/3 or L5/6 of input cortical areas, respectively. These layer-specific input patterns were largely independent of NMDA receptor function in the recipient neurons. mPFC L5 received proportionally more long-range inputs and more local inhibitory inputs than barrel cortex L5. Our results provide new insight into the organization and development of neocortical networks and identify important differences in the circuit organization in sensory and association cortices.
新皮质回路中的信息处理需要在广泛的空间尺度上整合输入信息，从局部微回路到长距离的皮质及皮质下连接。我们使用基于狂犬病病毒的跨突触示踪技术，来分析小鼠桶状皮质和内侧前额叶皮质（mPFC）中锥体细胞的局部和长距离输入的层状分布。在桶状皮质中，我们发现从第3层（L3）到第6层有大量输入，存在普遍的跨层抑制性输入，以及分别优先从输入皮质区域的L2/3或L5/6到L2/3或L5/6的长距离输入。这些层特异性输入模式在很大程度上独立于受体神经元中的NMDA受体功能。与桶状皮质L5相比，mPFC L5接受的长距离输入和成比例地更多的局部抑制性输入。我们的结果为新皮质网络的组织和发育提供了新的见解，并确定了感觉皮质和联合皮质在回路组织上的重要差异。
