Orazizadeh Mahmoud, Khodadadi Ali, Bayati Vahid, Saremy Sadegh, Farasat Maryam, Khorsandi Layasadat
Cell and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Immunology and Cancer, Petroleum Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Cell J. 2015 Fall;17(3):412-21. doi: 10.22074/cellj.2015.2. Epub 2015 Oct 7.
Zinc oxide nanoparticles (ZnO-NPs) are increasingly used in sunscreens, bio- sensors, food additives, pigments, manufacture of rubber products, and electronic materi- als. There are several studies about the effects of NPs on dermal fibroblast or keratino- cytes, but very little attention has been directed towards adipose-derived mesenchymal stem cells (ASCs). A previous study has revealed that ZnO-NPs restricted the migration capability of ASCs. However, the potential toxicity of these NPs on ASCs is not well un- derstood. This study intends to evaluate the effects of ZnO-NPs on subcutaneous ASCs.
In this experimental study, In order to assess toxicity, we ex- posed rat ASCs to ZnO-NPs at concentrations of 10, 50, and 100 µg/ml for 48 hours. Tox- icity was evaluated by cell morphology changes, cell viability assay, as well as apoptosis and necrosis detection.
ZnO-NPs concentration dependently reduced the survival rates of ASCs as re- vealed by the trypan blue exclusion and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazo- lium-bromide (MTT) tests. ZnO-NPs, at concentrations of 10 and 50 µg/ml, induced a significant increase in apoptotic indices as shown by the annexin V test. The concentration of 10 µg/ml of ZnO-NPs was more toxic.
Lower concentrations of ZnO-NPs have toxic and apoptotic effects on subcutaneous ASCs. We recommend that ZnO-NPs be used with caution if there is a dermatological problem.
氧化锌纳米颗粒（ZnO-NPs）越来越多地用于防晒霜、生物传感器、食品添加剂、颜料、橡胶制品制造和电子材料中。关于纳米颗粒对真皮成纤维细胞或角质形成细胞影响的研究有很多，但对脂肪来源的间充质干细胞（ASCs）的关注却很少。先前的一项研究表明，ZnO-NPs会限制ASCs的迁移能力。然而，这些纳米颗粒对ASCs的潜在毒性尚未得到充分了解。本研究旨在评估ZnO-NPs对皮下ASCs的影响。
在本实验研究中，为了评估毒性，我们将大鼠ASCs暴露于浓度为10、50和100μg/ml的ZnO-NPs中48小时。通过细胞形态变化、细胞活力测定以及凋亡和坏死检测来评估毒性。
锥虫蓝排斥试验和3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）试验表明，ZnO-NPs浓度依赖性地降低了ASCs的存活率。膜联蛋白V试验显示，浓度为10和50μg/ml的ZnO-NPs诱导凋亡指数显著增加。10μg/ml的ZnO-NPs毒性更大。
较低浓度的ZnO-NPs对皮下ASCs具有毒性和凋亡作用。我们建议，如果存在皮肤病问题，应谨慎使用ZnO-NPs。
