Azandeh Seyed Saeed, Abbaspour Mohammadreza, Khodadadi Ali, Khorsandi Layasadat, Orazizadeh Mahmoud, Heidari-Moghadam Abbas
Cell and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Pharm Res. 2017 Summer;16(3):868-879.
Curcumin (Cur) has been found to be very efficacious against many different types of cancer cells. However, the major disadvantage associated with the use of Cur is its low systemic bioavailability. Our present work investigated the toxic effect of encapsulation of Cur in PLGA (poly lactic-coglycolic acid) nanospheres (NCur) on PC3 human cancer prostate cell. In the present study, we have investigated the effects of NCur on growth, autophagia, and apoptosis in PC3 cells, respectively, by MTT assay, fluorescence microscopy, and Flow cytometry. MTT assays revealed that the NCur at the concentration of 25 µg/mL for 48 h were able to exert a more pronounced effect on the PC3 cells as compared to free Cur. Apoptotic index was significantly increased in NCur-treated cells compared to free Cur. The percentage of autophagic cells (LC3-II positive cells) was also significantly increased in NCur treatment in comparison to free Cur. These data indicate that the NCur has considerable cytotoxic activity more than Cur on PC3 cell lines, which is mediated by induction of both apoptotic and autophagic processes. Thus, NCur has high potential as an adjuvant therapy for clinical application in prostate cancer.
姜黄素（Cur）已被发现对许多不同类型的癌细胞非常有效。然而，使用Cur的主要缺点是其低全身生物利用度。我们目前的工作研究了将Cur包裹在聚乳酸-乙醇酸共聚物（PLGA）纳米球（NCur）中对PC3人前列腺癌细胞的毒性作用。在本研究中，我们分别通过MTT法、荧光显微镜和流式细胞术研究了NCur对PC3细胞生长、自噬和凋亡的影响。MTT分析表明，与游离Cur相比，浓度为25μg/mL的NCur作用48小时对PC3细胞的影响更显著。与游离Cur相比，NCur处理的细胞凋亡指数显著增加。与游离Cur相比，NCur处理中自噬细胞（LC3-II阳性细胞）的百分比也显著增加。这些数据表明，NCur对PC3细胞系具有比Cur更强的细胞毒性活性，这是通过诱导凋亡和自噬过程介导的。因此，NCur作为前列腺癌临床应用的辅助治疗具有很高的潜力。
