Knorr Roland L, Lipowsky Reinhard, Dimova Rumiana
a Max Planck Institute of Colloids and Interfaces; Science Park Golm ; Potsdam , Germany.
Autophagy. 2015 Nov 2;11(11):2134-2137. doi: 10.1080/15548627.2015.1091552.
During the intracellular process of macroautophagy (hereafter autophagy), a membrane-bound organelle, the autophagosome, is generated de novo. The remodeling of the autophagic membrane during the life cycle of the organelle is a complex multistep process and involves several changes in the topology of the autophagic membrane. Here, we focus on the final step of autophagosome formation, the closure of the phagophore, during which the inner and outer autophagic membranes become separate entities. We argue that this topological membrane transformation is a membrane scission event. Surprisingly, not a single recent review describes this substep as membrane scission (or membrane fission). In contrast, a number of publications imply that membrane fusion is involved. We discuss the potential sources for misinterpretation and recommend to consistent use of the unambiguous term "membrane scission."
在巨自噬（以下简称自噬）的细胞内过程中，一种膜结合细胞器——自噬体，会重新生成。在该细胞器的生命周期中，自噬膜的重塑是一个复杂的多步骤过程，涉及自噬膜拓扑结构的若干变化。在此，我们聚焦于自噬体形成的最后一步，即吞噬泡的封闭，在此过程中，自噬内膜和外膜成为独立的实体。我们认为这种拓扑膜转变是一种膜切割事件。令人惊讶的是，最近没有一篇综述将这一步骤描述为膜切割（或膜裂变）。相反，许多出版物暗示涉及膜融合。我们讨论了可能导致误解的来源，并建议始终使用明确无误的术语“膜切割”。
