酵母苯丙氨酸tRNA中被其同源合成酶特异性识别所需的核苷酸。
Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.
作者信息
Sampson J R, DiRenzo A B, Behlen L S, Uhlenbeck O C
机构信息
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
出版信息
Science. 1989 Mar 10;243(4896):1363-6. doi: 10.1126/science.2646717.
PMID:2646717
Abstract
An analysis of the aminoacylation kinetics of unmodified yeast tRNAPhe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.
摘要
对未修饰的酵母苯丙氨酸转运核糖核酸（tRNAPhe）突变体的氨酰化动力学分析表明，五个单链核苷酸对于酵母苯丙氨酰 - tRNA合成酶识别它很重要，前提是它们在正确折叠的tRNA结构中位置正确。当其他四种转运核糖核酸改变为具有这五个核苷酸时，它们就成为该酶近乎正常的底物。
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