Zheng Hong-Mei, Choi Myung-Joo, Kim Jae Min, Cha Kyung Hoi, Lee Kye Wan, Park Yu Hwa, Hong Soon-Sun, Lee Don Haeng
1 Department of New Drug Development, College of Medicine, Inha University Hospital , Incheon, Korea.
2 National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorder, Inha University Hospital , Incheon, Korea.
J Med Food. 2016 Jan;19(1):38-46. doi: 10.1089/jmf.2015.3464. Epub 2015 Oct 15.
The present study evaluated the protective effect of Centella asiatica (gotu kola) leaf extract (CAE) against indomethacin (IND)-induced gastric mucosal injury in rats. Gastric mucosal injury was induced by the oral administration of IND to the rats after a 24 h fast. CAE (50 or 250 mg/kg) or lansoprazole (a reference drug) was orally administrated 30 min before the IND administration, and 5 h later, the stomachs were removed to quantify the lesions. Orally administered CAE significantly reduced IND-induced gastric injury. The histopathological observations (hematoxylin-eosin and Periodic acid-Schiff staining) confirmed the protection against gastric mucosal injury. Also, CAE decreased the malondialdehyde content compared to the control group. Moreover, pretreatment with CAE resulted in a significant reduction in the elevated expression of tumor necrosis factor, Cyclooxygenase (COX)-2, and inducible nitric oxide synthase. These results suggested that CAE possesses gastroprotective effects against IND-induced gastric mucosal injury, which could be attributed to its ability to inhibit lipid peroxidation and stimulate gastric mucus secretion in the rat gastric mucosa.
本研究评估了积雪草叶提取物（CAE）对吲哚美辛（IND）诱导的大鼠胃黏膜损伤的保护作用。在大鼠禁食24小时后，通过口服IND诱导胃黏膜损伤。在给予IND前30分钟口服CAE（50或250mg/kg）或兰索拉唑（一种参考药物），5小时后取出胃以量化损伤。口服CAE可显著减轻IND诱导的胃损伤。组织病理学观察（苏木精-伊红染色和过碘酸-希夫染色）证实了对胃黏膜损伤的保护作用。此外，与对照组相比，CAE降低了丙二醛含量。此外，CAE预处理导致肿瘤坏死因子、环氧化酶（COX）-2和诱导型一氧化氮合酶的表达升高显著降低。这些结果表明，CAE对IND诱导的胃黏膜损伤具有胃保护作用，这可能归因于其抑制脂质过氧化和刺激大鼠胃黏膜分泌胃黏液的能力。
