Lv Lu, Peng Xinxin, Jing Shengli, Liu Bingfang, Zhu Lili, He Guangcun
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
J Econ Entomol. 2015 Aug;108(4):2021-9. doi: 10.1093/jee/tov122. Epub 2015 May 21.
Planthoppers in the genus Nilaparvata Distant are serious pests of rice and many other crops in tropical and temperate Asia, and northern Australia. In this study, the mitochondrial genomes of four Nilaparvata planthoppers were sequenced, three in Nilaparvata lugens Stål and one in Nilaparvata muiri China. Mitochondrial genome of Nilaparvata contain the standard set of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a control region. The nucleotide composition of Nilaparvata mitochondrial sequence is biased toward adenine and thymine, and the amino acid composition is affected to a similar degree by the bias to AT. We compare the four mitochondrial genomes and find intra- and interspecific variation in gene length, base composition, nucleotide and amino acid substitutions, intergenic spacer length, and gene overlap. The intra- and interspecific variations reveal that nucleotide and amino acid substitutions in mitochondrial protein-coding genes make a contribution to the formation of various insect biotypes in one species. Furthermore, the accumulation of nonsynonymous substitutions in the mitochondrial protein-coding genes, as well as differences in start codons, the length of intergenic spacers, and gene overlap regions contribute to differences between the two species investigated here. In addition, cox is the most conserved gene family and nad4-nad4l cluster is variable in Nilaparvata mitochondrial genes for the intra- and interspecific variation.
褐飞虱属（Nilaparvata Distant）的稻飞虱是热带和温带亚洲以及澳大利亚北部水稻和许多其他作物的严重害虫。在本研究中，对四种褐飞虱的线粒体基因组进行了测序，其中三种为褐飞虱（Nilaparvata lugens Stål），一种为穆氏褐飞虱（Nilaparvata muiri China）。褐飞虱的线粒体基因组包含13个标准的蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和一个控制区。褐飞虱线粒体序列的核苷酸组成偏向于腺嘌呤和胸腺嘧啶，氨基酸组成也受到类似程度的AT偏向影响。我们比较了这四个线粒体基因组，发现基因长度、碱基组成、核苷酸和氨基酸替换、基因间隔区长度以及基因重叠在种内和种间存在差异。种内和种间变异表明，线粒体蛋白质编码基因中的核苷酸和氨基酸替换有助于一个物种中各种昆虫生物型的形成。此外，线粒体蛋白质编码基因中非同义替换的积累，以及起始密码子、基因间隔区长度和基因重叠区域的差异，导致了这里研究的两个物种之间的差异。此外，cox是最保守的基因家族，nad4 - nad4l簇在褐飞虱线粒体基因的种内和种间变异中是可变的。
