Park Sooyeon, Jung Yong-Taek, Park Ji-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3231-3236. doi: 10.1099/ijsem.0.002091. Epub 2017 Aug 22.
A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped or ovoid bacterium, designated strain KEM-10T, was isolated from water in an estuary environment in the Yellow Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain KEM-10T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain KEM-10T belonged to the genus Algoriphagus, joining the type strain of Algoriphagus litorisediminis showing 97.4 % sequence similarity. Strain KEM-10T exhibited 16S rRNA gene sequence similarity of 92.2-96.1 % to the type strains of the other Algoriphagus species. Strain KEM-10T contained MK-7 as the predominant menaquinone and iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major fatty acids. The major polar lipids detected in strain KEM-10T were phosphatidylethanolamine and phosphatidylcholine. The DNA G+C content of strain KEM-10T was 40.6 mol%. The mean DNA-DNA relatedness value between strain KEM-10T and the type strain of A. litorisediminis was 12 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain KEM-10T is separated from recognized species of the genus Algoriphagus. On the basis of the data presented, strain KEM-10T is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus aquaemixtae sp. nov. is proposed. The type strain is KEM-10T (=KCTC 52839T=NBRC 112780T).
从韩国黄海河口环境的水中分离出一株革兰氏阴性、无鞭毛、不滑动、需氧的杆状或卵形细菌，命名为KEM-10T菌株，并对其进行了多相分类研究。KEM-10T菌株在25 °C、pH 7.0 - 8.0和2.0 %（w/v）NaCl条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树显示，KEM-10T菌株属于嗜冷杆菌属，与海滨嗜冷杆菌模式菌株的序列相似性为97.4 %。KEM-10T菌株与其他嗜冷杆菌属物种的模式菌株的16S rRNA基因序列相似性为92.2 - 96.1 %。KEM-10T菌株以MK-7作为主要甲基萘醌，以异-C15 : 0和特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）作为主要脂肪酸。在KEM-10T菌株中检测到的主要极性脂质是磷脂酰乙醇胺和磷脂酰胆碱。KEM-10T菌株的DNA G+C含量为40.6 mol%。KEM-10T菌株与海滨嗜冷杆菌模式菌株之间的平均DNA-DNA相关性值为12 %。不同的表型特征，以及其系统发育和遗传独特性，表明KEM-10T菌株与已确认的嗜冷杆菌属物种不同。根据所提供的数据，KEM-10T菌株被认为代表嗜冷杆菌属的一个新物种，为此提出名为Algoriphagus aquaemixtae sp. nov.的新名称。模式菌株为KEM-10T（=KCTC 52839T=NBRC 112780T）。
