Krasowski Grzegorz, Junka Adam, Paleczny Justyna, Czajkowska Joanna, Makomaska-Szaroszyk Elżbieta, Chodaczek Grzegorz, Majkowski Michał, Migdał Paweł, Fijałkowski Karol, Kowalska-Krochmal Beata, Bartoszewicz Marzenna
Nutrikon, KCZ Surgical Ward, 47-300 Krapkowice, Poland.
Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wrocław Medical University, 50-556 Wrocław, Poland.
Membranes (Basel). 2021 Jan 17;11(1):62. doi: 10.3390/membranes11010062.
Chronic wounds complicated with biofilm formed by pathogens remain one of the most significant challenges of contemporary medicine. The application of topical antiseptic solutions against wound biofilm has been gaining increasing interest among clinical practitioners and scientific researchers. This paper compares the activity of polyhexanide-, octenidine- and hypochlorite/hypochlorous acid-based antiseptics against biofilm formed by clinical strains of and . The analyses included both standard techniques utilizing polystyrene plates and self-designed biocellulose-based models in which a biofilm formed by pathogens was formed on an elastic, fibrinous surface covered with a fibroblast layer. The obtained results show high antibiofilm activity of polihexanide- and octenidine-based antiseptics and lack or weak antibiofilm activity of hypochlorite-based antiseptic of total chlorine content equal to 80 parts per million. The data presented in this paper indicate that polihexanide- or octenidine-based antiseptics are highly useful in the treatment of biofilm, while hypochlorite-based antiseptics with low chlorine content may be applied for wound rinsing but not when specific antibiofilm activity is required.
由病原体形成生物膜的慢性伤口仍然是当代医学面临的最重大挑战之一。局部应用抗菌溶液对抗伤口生物膜在临床医生和科研人员中越来越受到关注。本文比较了聚己缩胍、奥替尼啶和次氯酸盐/次氯酸类抗菌剂对由临床分离株和形成的生物膜的活性。分析包括利用聚苯乙烯板的标准技术和自行设计的基于生物纤维素的模型，在该模型中，病原体形成的生物膜在覆盖有成纤维细胞层的弹性纤维表面形成。所得结果表明，聚己缩胍类和奥替尼啶类抗菌剂具有较高的抗生物膜活性，而总氯含量等于百万分之八十的次氯酸盐类抗菌剂则缺乏或具有较弱的抗生物膜活性。本文提供的数据表明，聚己缩胍类或奥替尼啶类抗菌剂在生物膜治疗中非常有用，而低氯含量的次氯酸盐类抗菌剂可用于伤口冲洗，但在需要特定抗生物膜活性时则不适用。
