[一测多评法测定栀子中6种成分的含量]
[Determination of Six Ingredients in Gardenia jaminoides Fruits with Quantitative Analysis of Muti-components by Single Marker].
作者信息
Lin Shao-xia, Zhang Qing-hai, Xi Pei-yu, Lin Chang-hu, Xiao Zi-fan
出版信息
Zhong Yao Cai. 2015 Mar;38(3):531-5.
OBJECTIVE
To establish a method of quantitative analysis of multi-components, by single marker(QAMS)for simultaneously determining six ingredients in Gardenia jasminoides fruits.
METHODS
A multi-wavelength segmentation detection method was used. A methodological mode was found to analysis six ingredients in Gardenia jasminoides fruits by quantitative analysis of QAMS. Taken geniposide as reference to create RCF with gardenia acid, chlorogenic acid, crocin I, crocin II and crocin III.
RESULTS
The good reproducibility and acceptable durability of method was validated between two HPLC systems and three columns. 20 batches of Gardenia jaminoides fruits was analysis, and the results showed good linear correlation compared to external standard method (r > 0. 999).
CONCLUSION
QAMS can be used as quality evaluation method of multi-component Gardenia jaminoides fruits.
目的
建立一种多成分单指标定量分析（QAMS）方法，用于同时测定栀子果实中的六种成分。
方法
采用多波长分段检测法。通过QAMS定量分析，找到一种分析栀子果实中六种成分的方法模式。以栀子苷为对照品，与栀子酸、绿原酸、西红花苷I、西红花苷II和西红花苷III建立相对校正因子（RCF）。
结果
在两个高效液相色谱（HPLC）系统和三根色谱柱之间验证了该方法具有良好的重复性和可接受的耐用性。对20批栀子果实进行分析，结果表明与外标法相比具有良好的线性相关性（r>0.999）。
结论
QAMS可作为多成分栀子果实的质量评价方法。
Zotero 插件