Huang Ziling, Zhang Long, Yi Xianghua, Yu Xiaoting
Department of Pathology, Tongji Hospital, Tongji University School of Medicine, No. 389 Xincun Road, Shanghai, 200065, China.
Department of Pathology, Labway Clinical Laboratory Shanghai Ltd, Shanghai, 200000, China.
Tumour Biol. 2016 Apr;37(4):4359-65. doi: 10.1007/s13277-015-4262-9. Epub 2015 Oct 24.
Prostate cancer (PCa) has become a prevalent malignant disease in males globally. Accumulating data suggested that hsa-microRNAs (miRNAs) could be potential biomarkers for tumor diagnosis due to their important roles in the cell cycle. This study investigated the diagnostic and prognostic values of hsa-miR-203 and hsa-miR-30c in PCa tissues. There were 44 pathologically confirmed PCa patients who were enrolled in this study. Tissue samples were collected from both tumor tissues and adjacent normal tissues. RNA was extracted and the expression levels of hsa-miR-203 and hsa-miR-30c in tumor and normal tissues were compared. The receiver operating characteristic (ROC) curves were plotted to evaluate the reliability of hsa-miR-203 and hsa-miR-30c in detecting PCa. All subjects in this study were followed up by 36 months, and the Kaplan-Meier method was conducted to investigate the survival status of PCa patients. The average relative expressions of hsa-miR-203 and hsa-miR-30c in tumor tissues were significantly different from those in adjacent normal tissues (P < 0.001), and the predictive power of the two hsa-miRNAs for PCa prognosis was reliable. Besides that, the average survival times of low-hsa-miR-30c and high-hsa-miR-203 groups were significantly lower than those of the corresponding groups with the log-rank P of 0.015 and 0.023, respectively. In summary, our study suggested that both hsa-miR-203 and hsa-miR-30c are potential biomarkers for detection and prognosis of PCa.
前列腺癌（PCa）已成为全球男性中一种普遍的恶性疾病。越来越多的数据表明，由于人源微小RNA（miRNA）在细胞周期中发挥重要作用，它们可能成为肿瘤诊断的潜在生物标志物。本研究调查了人源miR-203和人源miR-30c在PCa组织中的诊断和预后价值。本研究纳入了44例经病理确诊的PCa患者。从肿瘤组织和相邻正常组织中采集组织样本。提取RNA并比较肿瘤组织和正常组织中人源miR-203和人源miR-30c的表达水平。绘制受试者工作特征（ROC）曲线以评估人源miR-203和人源miR-30c检测PCa的可靠性。本研究中的所有受试者均随访36个月，并采用Kaplan-Meier方法调查PCa患者的生存状况。肿瘤组织中人源miR-203和人源miR-30c的平均相对表达与相邻正常组织中的平均相对表达有显著差异（P < 0.001），且这两种人源miRNA对PCa预后的预测能力可靠。此外，低人源miR-30c组和高人源miR-203组的平均生存时间显著低于相应组，对数秩检验P值分别为0.015和0.023。总之，我们的研究表明，人源miR-203和人源miR-30c均是PCa检测和预后的潜在生物标志物。
