Heris Hossein K, Daoud Jamal, Sheibani Sara, Vali Hojatollah, Tabrizian Maryam, Mongeau Luc
Biomechanics Laboratory, Department of Mechanical Engineering, Faculty of Engineering, McGill University, 817 Rue Sherbrooke Ouest, Montreal, QC, H3A 0C3, Canada.
Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada.
Adv Healthc Mater. 2016 Jan 21;5(2):255-65. doi: 10.1002/adhm.201500370. Epub 2015 Oct 26.
The potential use of a novel scaffold biomaterial consisting of cross-linked hyaluronic acid (HA)-gelatin (Ge) composite microgels is investigated for use in treating vocal fold injury and scarring. Cell adhesion integrins and kinematics of cell motion are investigated in 2D and 3D culture conditions, respectively. Human vocal fold fibroblast (hVFF) cells are seeded on HA-Ge microgels attached to a HA hydrogel thin film. The results show that hVFF cells establish effective adhesion to HA-Ge microgels through the ubiquitous expression of β1 integrin in the cell membrane. The microgels are then encapsulated in a 3D HA hydrogel for the study of cell migration. The cells within the HA-Ge microgel-reinforced composite hydrogel (MRCH) scaffold have an average motility speed of 0.24 ± 0.08 μm min(-1) . The recorded microscopic images reveal features that are presumably associated with lobopodial and lamellipodial cell migration modes within the MRCH scaffold. Average cell speed during lobopodial migration is greater than that during lamellipodial migration. The cells move faster in the MRCH than in the HA-Ge gel without microgels. These findings support the hypothesis that HA-Ge MRCH promotes cell adhesion and migration; thereby they constitute a promising biomaterial for vocal fold repair.
研究了一种由交联透明质酸（HA）-明胶（Ge）复合微凝胶组成的新型支架生物材料在治疗声带损伤和瘢痕形成中的潜在应用。分别在二维和三维培养条件下研究了细胞粘附整合素和细胞运动学。将人声带成纤维细胞（hVFF）接种在附着于HA水凝胶薄膜的HA-Ge微凝胶上。结果表明，hVFF细胞通过细胞膜中β1整合素的普遍表达与HA-Ge微凝胶建立有效粘附。然后将微凝胶封装在三维HA水凝胶中以研究细胞迁移。HA-Ge微凝胶增强复合水凝胶（MRCH）支架内的细胞平均运动速度为0.24±0.08μm min(-1)。记录的显微镜图像揭示了可能与MRCH支架内叶足状和片状伪足细胞迁移模式相关的特征。叶足状迁移期间的平均细胞速度大于片状伪足迁移期间的平均细胞速度。细胞在MRCH中比在没有微凝胶的HA-Ge凝胶中移动得更快。这些发现支持了HA-Ge MRCH促进细胞粘附和迁移的假设；因此，它们构成了一种有前途的声带修复生物材料。
