若丹明B作为一种光学温度计，用于局部暴露于红外激光或纳秒脉冲电场的细胞中。
Rhodamine B as an optical thermometer in cells focally exposed to infrared laser light or nanosecond pulsed electric fields.
作者信息
Moreau David, Lefort Claire, Burke Ryan, Leveque Philippe, O'Connor Rodney P
机构信息
Univ. Limoges, CNRS, XLIM, UMR 7252, F-87000 Limoges, France.
出版信息
Biomed Opt Express. 2015 Sep 24;6(10):4105-17. doi: 10.1364/BOE.6.004105. eCollection 2015 Oct 1.
Abstract
The temperature-dependent fluorescence property of Rhodamine B was used to measure changes in temperature at the cellular level induced by either infrared laser light exposure or high intensity, ultrashort pulsed electric fields. The thermal impact of these stimuli were demonstrated at the cellular level in time and contrasted with the change in temperature observed in the extracellular bath. The method takes advantage of the temperature sensitivity of the fluorescent dye Rhodamine B which has a quantum yield linearly dependent on temperature. The thermal effects of different temporal pulse applications of infrared laser light exposure and of nanosecond pulsed electric fields were investigated. The temperature increase due to the application of nanosecond pulsed electric fields was demonstrated at the cellular level.
摘要
罗丹明B的温度依赖性荧光特性被用于测量在细胞水平上由红外激光照射或高强度超短脉冲电场引起的温度变化。这些刺激的热效应在细胞水平上及时得到了证实，并与细胞外浴中观察到的温度变化进行了对比。该方法利用了荧光染料罗丹明B的温度敏感性，其量子产率与温度呈线性相关。研究了红外激光照射和纳秒脉冲电场不同时间脉冲应用的热效应。在细胞水平上证实了纳秒脉冲电场应用导致的温度升高。
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