el-Ghorr A A, Snodgrass D R, Scott F M, Campbell I
Moredun Research Institute, Edinburgh, Scotland.
Arch Virol. 1989;104(3-4):241-8. doi: 10.1007/BF01315546.
Two bovine coronavirus (BCV) strains from diarrheic calf faeces were adapted to grow in HRT 18 cells and compared in immunofluorescence (IF), haemagglutination inhibition (HAI) and neutralisation (NT) tests with three other strains of BCV and a human coronavirus (HCV) strain obtained from other laboratories. Polyclonal antisera against these 6 viruses were raised in rabbits. No significant differences between viruses were detected by IF. In the HAI test the HCV strain was distinguishable from the 5 BCV strains and serological variation between the BCV strains was shown. HCV could be distinguished by NT test, but all BCV isolates were similar. Two monoclonal antibodies prepared against one of the BCV strains distinguished HCV in all three assays, and detected varying relationships between BCV strains.
从腹泻小牛粪便中分离出的两株牛冠状病毒(BCV)毒株,经适应后可在HRT 18细胞中生长,并与从其他实验室获得的另外三株BCV毒株及一株人冠状病毒(HCV)毒株进行免疫荧光(IF)、血凝抑制(HAI)和中和(NT)试验比较。用这些病毒对家兔进行免疫,制备了针对这6种病毒的多克隆抗血清。IF试验未检测到病毒之间的显著差异。在HAI试验中,HCV毒株与5株BCV毒株有区别,且显示出BCV毒株之间的血清学差异。NT试验可区分HCV,但所有BCV分离株相似。针对其中一株BCV毒株制备的两种单克隆抗体在所有三种试验中均可区分HCV,并检测到BCV毒株之间的不同关系。