Zhu Ya, Su Guanyong, Yang Dandong, Zhang Yongkang, Yu Liqin, Li Yufei, Giesy John P, Letcher Robert J, Liu Chunsheng
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
Environ Pollut. 2017 Oct;229:470-478. doi: 10.1016/j.envpol.2017.06.024. Epub 2017 Jun 15.
Growth curves were used to determine sensitive exposure windows for evaluation of developmental toxicity of chemicals to zebrafish. Dose- and time-dependent effects on body mass, body length and expression of genes involved in the growth hormone/insulin-like growth factor (GH/IGF) axis and the hypothalamic-pituitary-thyroid (HPT) axis were examined after exposure to environmentally relevant concentrations of tris(1,3-dichloro-2-propyl) phosphate (TDCIPP). Based on growth curves, zebrafish grew most rapidly between 60 and 90 days post fertilization (dpf). Exposure to environmentally relevant concentrations of TDCIPP significantly decreased body mass and body length and down-regulated expression of several genes involved in the GH/IGF axis of female zebrafish, but no such effects were observed in male zebrafish. Exposure to TDCIPP did not change concentrations of thyroid hormones or expression of genes along the HPT axis in female and male zebrafish. These results suggest that growth stages of zebrafish between 60 and 90 dpf might be most appropriate for evaluation of developmental toxicity of chemicals, and down-regulation of genes involved in the GH/IGF axis, but not the HPT axis, might be responsible for the observed growth inhibition in females exposed to TDCIPP.
生长曲线用于确定敏感暴露窗口，以评估化学物质对斑马鱼的发育毒性。在暴露于环境相关浓度的磷酸三（1,3 - 二氯 - 2 - 丙基）酯（TDCIPP）后，检测了对体重、体长以及生长激素/胰岛素样生长因子（GH/IGF）轴和下丘脑 - 垂体 - 甲状腺（HPT）轴相关基因表达的剂量和时间依赖性影响。基于生长曲线，斑马鱼在受精后60至90天（dpf）生长最为迅速。暴露于环境相关浓度的TDCIPP显著降低了雌性斑马鱼的体重和体长，并下调了GH/IGF轴相关的几个基因的表达，但在雄性斑马鱼中未观察到此类影响。暴露于TDCIPP并未改变雌性和雄性斑马鱼的甲状腺激素浓度或HPT轴相关基因的表达。这些结果表明，斑马鱼60至90 dpf的生长阶段可能最适合评估化学物质的发育毒性，并且GH/IGF轴相关基因的下调而非HPT轴相关基因的下调可能是导致暴露于TDCIPP的雌性斑马鱼生长受抑制的原因。
