用线性化质粒对大肠杆菌recD菌株进行染色体转化。
Chromosomal transformation of Escherichia coli recD strains with linearized plasmids.
作者信息
Russell C B, Thaler D S, Dahlquist F W
机构信息
Institute of Molecular Biology, University of Oregon, Eugene 97403.
出版信息
J Bacteriol. 1989 May;171(5):2609-13. doi: 10.1128/jb.171.5.2609-2613.1989.
Abstract
Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.
摘要
野生型大肠杆菌对纯化的线性DNA介导的遗传转化具有抗性，这可能部分归因于核酸外切酶活性。我们证明，含有recD突变的大肠杆菌能够被携带选择标记的线性化质粒轻易转化。该标记通过同源重组转移至染色体，而位于同源区域之外的质粒标记则会丢失。
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